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A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens

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  • Chen Li

    (National Institute for Basic Biology
    School of Life Science, SOKENDAI (The Graduate University for Advanced Studies)
    Present address: School of Pharmacy, Hubei University of Medicine, Shiyan city, Hubei 442000, China)

  • Yusuke Sako

    (National Institute for Basic Biology
    ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency)

  • Akihiro Imai

    (National Institute for Basic Biology
    ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency
    Present address: Faculty of Life Sciences, Hiroshima Institute of Technology, Hiroshima 731-5193, Japan)

  • Tomoaki Nishiyama

    (ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency
    Advanced Science Research Center, Institute for Gene Research, Kanazawa University)

  • Kari Thompson

    (National Institute for Basic Biology
    ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency
    West Virginia University)

  • Minoru Kubo

    (National Institute for Basic Biology
    ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency
    Present address: Institute for Research Initiatives, Nara Institute of Science and Technology, Nara 630-1092, Japan)

  • Yuji Hiwatashi

    (National Institute for Basic Biology
    School of Life Science, SOKENDAI (The Graduate University for Advanced Studies)
    Present address: School of Food, Agricultural and Environmental Sciences, Miyagi University, Sendai 982-0215, Japan)

  • Yukiko Kabeya

    (National Institute for Basic Biology)

  • Dale Karlson

    (West Virginia University)

  • Shu-Hsing Wu

    (Institute of Plant and Microbial Biology, Academia Sinica)

  • Masaki Ishikawa

    (National Institute for Basic Biology
    School of Life Science, SOKENDAI (The Graduate University for Advanced Studies))

  • Takashi Murata

    (National Institute for Basic Biology
    School of Life Science, SOKENDAI (The Graduate University for Advanced Studies))

  • Philip N. Benfey

    (Duke University)

  • Yoshikatsu Sato

    (National Institute for Basic Biology
    ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency
    Present address: Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya 464-8602, Japan)

  • Yosuke Tamada

    (National Institute for Basic Biology
    School of Life Science, SOKENDAI (The Graduate University for Advanced Studies))

  • Mitsuyasu Hasebe

    (National Institute for Basic Biology
    School of Life Science, SOKENDAI (The Graduate University for Advanced Studies)
    ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency)

Abstract

Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3′-untranslated region (3′-UTR). Removal of the 3′-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28.

Suggested Citation

  • Chen Li & Yusuke Sako & Akihiro Imai & Tomoaki Nishiyama & Kari Thompson & Minoru Kubo & Yuji Hiwatashi & Yukiko Kabeya & Dale Karlson & Shu-Hsing Wu & Masaki Ishikawa & Takashi Murata & Philip N. Ben, 2017. "A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens," Nature Communications, Nature, vol. 8(1), pages 1-13, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14242
    DOI: 10.1038/ncomms14242
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