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Temporal and compartment-specific signals coordinate mitotic exit with spindle position

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  • Ayse Koca Caydasi

    (DKFZ-ZMBH Alliance, German Cancer Research Centre (DKFZ)
    Centre for Organismal Studies (COS), University of Heidelberg
    Present address: Department of Molecular Biology and Genetics, Gebze Technical University, 41400 Gebze/Kocaeli, Turkey)

  • Anton Khmelinskii

    (DKFZ-ZMBH Alliance, Centre for Molecular Biology (ZMBH), University of Heidelberg)

  • Rafael Duenas-Sanchez

    (DKFZ-ZMBH Alliance, German Cancer Research Centre (DKFZ)
    Centre for Organismal Studies (COS), University of Heidelberg)

  • Bahtiyar Kurtulmus

    (DKFZ-ZMBH Alliance, German Cancer Research Centre (DKFZ)
    Centre for Organismal Studies (COS), University of Heidelberg)

  • Michael Knop

    (DKFZ-ZMBH Alliance, German Cancer Research Centre (DKFZ)
    DKFZ-ZMBH Alliance, Centre for Molecular Biology (ZMBH), University of Heidelberg)

  • Gislene Pereira

    (DKFZ-ZMBH Alliance, German Cancer Research Centre (DKFZ)
    Centre for Organismal Studies (COS), University of Heidelberg)

Abstract

The spatiotemporal control of mitotic exit is crucial for faithful chromosome segregation during mitosis. In budding yeast, the mitotic exit network (MEN) drives cells out of mitosis, whereas the spindle position checkpoint (SPOC) blocks MEN activity when the anaphase spindle is mispositioned. How the SPOC operates at a molecular level remains unclear. Here, we report novel insights into how mitotic signalling pathways orchestrate chromosome segregation in time and space. We establish that the key function of the central SPOC kinase, Kin4, is to counterbalance MEN activation by the cdc fourteen early anaphase release (FEAR) network in the mother cell compartment. Remarkably, Kin4 becomes dispensable for SPOC function in the absence of FEAR. Cells lacking both FEAR and Kin4 show that FEAR contributes to mitotic exit through regulation of the SPOC component Bfa1 and the MEN kinase Cdc15. Furthermore, we uncover controls that specifically promote mitotic exit in the daughter cell compartment.

Suggested Citation

  • Ayse Koca Caydasi & Anton Khmelinskii & Rafael Duenas-Sanchez & Bahtiyar Kurtulmus & Michael Knop & Gislene Pereira, 2017. "Temporal and compartment-specific signals coordinate mitotic exit with spindle position," Nature Communications, Nature, vol. 8(1), pages 1-14, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14129
    DOI: 10.1038/ncomms14129
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