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Temporal stability and molecular persistence of the bone marrow plasma cell antibody repertoire

Author

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  • Gabriel C. Wu

    (Center for Systems and Synthetic Biology, University of Texas at Austin)

  • Nai-Kong V. Cheung

    (Memorial Sloan-Kettering Cancer Center, New York)

  • George Georgiou

    (University of Texas at Austin
    University of Texas at Austin
    University of Texas at Austin
    Institute for Cell and Molecular Biology, University of Texas at Austin)

  • Edward M. Marcotte

    (Center for Systems and Synthetic Biology, University of Texas at Austin
    University of Texas at Austin
    Institute for Cell and Molecular Biology, University of Texas at Austin)

  • Gregory C. Ippolito

    (University of Texas at Austin)

Abstract

Plasma cells in human bone marrow (BM) are thought to be responsible for sustaining lifelong immunity, but its underlying basis is controversial. Here we use high-throughput sequence analysis of the same individual across 6.5 years to show that the BM plasma cell immunoglobulin heavy chain repertoire is remarkably stable over time. We find a nearly static bias in individual and combinatorial gene usage across time. Analysis of a second donor corroborates these observations. We also report the persistence of numerous BM plasma cell clonotypes (∼2%) identifiable at all points assayed across 6.5 years, supporting a model of serological memory based upon intrinsic longevity of human plasma cells. Donors were adolescents who completely recovered from neuroblastoma prior to the start of this study. Our work will facilitate differentiation between healthy and diseased antibody repertoires, by serving as a point of comparison with future deep-sequencing studies involving immune intervention.

Suggested Citation

  • Gabriel C. Wu & Nai-Kong V. Cheung & George Georgiou & Edward M. Marcotte & Gregory C. Ippolito, 2016. "Temporal stability and molecular persistence of the bone marrow plasma cell antibody repertoire," Nature Communications, Nature, vol. 7(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13838
    DOI: 10.1038/ncomms13838
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