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A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

Author

Listed:
  • Patrick M. Shih

    (Joint BioEnergy Institute, Emery Station East
    Lawrence Berkeley National Laboratory)

  • Khanh Vuu

    (Joint BioEnergy Institute, Emery Station East
    Lawrence Berkeley National Laboratory)

  • Nasim Mansoori

    (Joint BioEnergy Institute, Emery Station East
    Lawrence Berkeley National Laboratory)

  • Leïla Ayad

    (Joint BioEnergy Institute, Emery Station East
    Lawrence Berkeley National Laboratory)

  • Katherine B. Louie

    (Lawrence Berkeley National Laboratory
    Joint Genome Institute)

  • Benjamin P. Bowen

    (Lawrence Berkeley National Laboratory
    Joint Genome Institute)

  • Trent R. Northen

    (Joint BioEnergy Institute, Emery Station East
    Lawrence Berkeley National Laboratory
    Joint Genome Institute)

  • Dominique Loqué

    (Joint BioEnergy Institute, Emery Station East
    Lawrence Berkeley National Laboratory
    Université Claude Bernard Lyon 1, INSA de Lyon, CNRS, UMR5240, Microbiologie, Adaptation et Pathogénie)

Abstract

The advent and growth of synthetic biology has demonstrated its potential as a promising avenue of research to address many societal needs. However, plant synthetic biology efforts have been hampered by a dearth of DNA part libraries, versatile transformation vectors and efficient assembly strategies. Here, we describe a versatile system (named jStack) utilizing yeast homologous recombination to efficiently assemble DNA into plant transformation vectors. We demonstrate how this method can facilitate pathway engineering of molecules of pharmaceutical interest, production of potential biofuels and shuffling of disease-resistance traits between crop species. Our approach provides a powerful alternative to conventional strategies for stacking genes and traits to address many impending environmental and agricultural challenges.

Suggested Citation

  • Patrick M. Shih & Khanh Vuu & Nasim Mansoori & Leïla Ayad & Katherine B. Louie & Benjamin P. Bowen & Trent R. Northen & Dominique Loqué, 2016. "A robust gene-stacking method utilizing yeast assembly for plant synthetic biology," Nature Communications, Nature, vol. 7(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13215
    DOI: 10.1038/ncomms13215
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