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A modular platform for one-step assembly of multi-component membrane systems by fusion of charged proteoliposomes

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  • Robert R. Ishmukhametov

    (University of Oxford, Clarendon Laboratory)

  • Aidan N. Russell

    (University of Oxford, Clarendon Laboratory)

  • Richard M. Berry

    (University of Oxford, Clarendon Laboratory)

Abstract

An important goal in synthetic biology is the assembly of biomimetic cell-like structures, which combine multiple biological components in synthetic lipid vesicles. A key limiting assembly step is the incorporation of membrane proteins into the lipid bilayer of the vesicles. Here we present a simple method for delivery of membrane proteins into a lipid bilayer within 5 min. Fusogenic proteoliposomes, containing charged lipids and membrane proteins, fuse with oppositely charged bilayers, with no requirement for detergent or fusion-promoting proteins, and deliver large, fragile membrane protein complexes into the target bilayers. We demonstrate the feasibility of our method by assembling a minimal electron transport chain capable of adenosine triphosphate (ATP) synthesis, combining Escherichia coli F1Fo ATP-synthase and the primary proton pump bo3-oxidase, into synthetic lipid vesicles with sizes ranging from 100 nm to ∼10 μm. This provides a platform for the combination of multiple sets of membrane protein complexes into cell-like artificial structures.

Suggested Citation

  • Robert R. Ishmukhametov & Aidan N. Russell & Richard M. Berry, 2016. "A modular platform for one-step assembly of multi-component membrane systems by fusion of charged proteoliposomes," Nature Communications, Nature, vol. 7(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13025
    DOI: 10.1038/ncomms13025
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