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Two-dimensional flow nanometry of biological nanoparticles for accurate determination of their size and emission intensity

Author

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  • Stephan Block

    (Chalmers University of Technology
    Present address: Department of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany.)

  • Björn Johansson Fast

    (Chalmers University of Technology)

  • Anders Lundgren

    (Chalmers University of Technology
    University of Natural Resources and Life Sciences)

  • Vladimir P. Zhdanov

    (Chalmers University of Technology
    Boreskov Institute of Catalysis, Russian Academy of Sciences)

  • Fredrik Höök

    (Chalmers University of Technology)

Abstract

Biological nanoparticles (BNPs) are of high interest due to their key role in various biological processes and use as biomarkers. BNP size and composition are decisive for their functions, but simultaneous determination of both properties with high accuracy remains challenging. Optical microscopy allows precise determination of fluorescence/scattering intensity, but not the size of individual BNPs. The latter is better determined by tracking their random motion in bulk, but the limited illumination volume for tracking this motion impedes reliable intensity determination. Here, we show that by attaching BNPs to a supported lipid bilayer, subjecting them to hydrodynamic flows and tracking their motion via surface-sensitive optical imaging enable determination of their diffusion coefficients and flow-induced drifts, from which accurate quantification of both BNP size and emission intensity can be made. For vesicles, the accuracy of this approach is demonstrated by resolving the expected radius-squared dependence of their fluorescence intensity for radii down to 15 nm.

Suggested Citation

  • Stephan Block & Björn Johansson Fast & Anders Lundgren & Vladimir P. Zhdanov & Fredrik Höök, 2016. "Two-dimensional flow nanometry of biological nanoparticles for accurate determination of their size and emission intensity," Nature Communications, Nature, vol. 7(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12956
    DOI: 10.1038/ncomms12956
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