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Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care

Author

Listed:
  • Pablo Perez-Pinera

    (Synthetic Biology Group, Massachusetts Institute of Technology
    Research Laboratory of Electronics, Massachusetts Institute of Technology
    Present address: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.)

  • Ningren Han

    (Research Laboratory of Electronics, Massachusetts Institute of Technology)

  • Sara Cleto

    (Synthetic Biology Group, Massachusetts Institute of Technology
    Research Laboratory of Electronics, Massachusetts Institute of Technology)

  • Jicong Cao

    (Synthetic Biology Group, Massachusetts Institute of Technology
    Research Laboratory of Electronics, Massachusetts Institute of Technology)

  • Oliver Purcell

    (Synthetic Biology Group, Massachusetts Institute of Technology
    Research Laboratory of Electronics, Massachusetts Institute of Technology)

  • Kartik A. Shah

    (The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology)

  • Kevin Lee

    (Pharyx Inc.)

  • Rajeev Ram

    (Research Laboratory of Electronics, Massachusetts Institute of Technology)

  • Timothy K. Lu

    (Synthetic Biology Group, Massachusetts Institute of Technology
    Research Laboratory of Electronics, Massachusetts Institute of Technology
    The Broad Institute of MIT and Harvard)

Abstract

Current biopharmaceutical manufacturing systems are not compatible with portable or distributed production of biologics, as they typically require the development of single biologic-producing cell lines followed by their cultivation at very large scales. Therefore, it remains challenging to treat patients in short time frames, especially in remote locations with limited infrastructure. To overcome these barriers, we developed a platform using genetically engineered Pichia pastoris strains designed to secrete multiple proteins on programmable cues in an integrated, benchtop, millilitre-scale microfluidic device. We use this platform for rapid and switchable production of two biologics from a single yeast strain as specified by the operator. Our results demonstrate selectable and near-single-dose production of these biologics in

Suggested Citation

  • Pablo Perez-Pinera & Ningren Han & Sara Cleto & Jicong Cao & Oliver Purcell & Kartik A. Shah & Kevin Lee & Rajeev Ram & Timothy K. Lu, 2016. "Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care," Nature Communications, Nature, vol. 7(1), pages 1-10, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12211
    DOI: 10.1038/ncomms12211
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    Cited by:

    1. Masahiro Tominaga & Yoko Shima & Kenta Nozaki & Yoichiro Ito & Masataka Someda & Yuji Shoya & Noritaka Hashii & Chihiro Obata & Miho Matsumoto-Kitano & Kohei Suematsu & Tadashi Matsukawa & Keita Hosoy, 2024. "Designing strong inducible synthetic promoters in yeasts," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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