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Whole-animal genome-wide RNAi screen identifies networks regulating male germline stem cells in Drosophila

Author

Listed:
  • Ying Liu

    (Basic Research Laboratory, National Cancer Institute at Frederick, National Institutes of Health)

  • Qinglan Ge

    (Basic Research Laboratory, National Cancer Institute at Frederick, National Institutes of Health)

  • Brian Chan

    (Basic Research Laboratory, National Cancer Institute at Frederick, National Institutes of Health)

  • Hanhan Liu

    (Basic Research Laboratory, National Cancer Institute at Frederick, National Institutes of Health)

  • Shree Ram Singh

    (Basic Research Laboratory, National Cancer Institute at Frederick, National Institutes of Health)

  • Jacob Manley

    (Basic Research Laboratory, National Cancer Institute at Frederick, National Institutes of Health)

  • Jae Lee

    (Basic Research Laboratory, National Cancer Institute at Frederick, National Institutes of Health)

  • Ann Marie Weideman

    (Basic Research Laboratory, National Cancer Institute at Frederick, National Institutes of Health)

  • Gerald Hou

    (Basic Research Laboratory, National Cancer Institute at Frederick, National Institutes of Health)

  • Steven X. Hou

    (Basic Research Laboratory, National Cancer Institute at Frederick, National Institutes of Health)

Abstract

Stem cells are regulated both intrinsically and externally, including by signals from the local environment and distant organs. To identify genes and pathways that regulate stem-cell fates in the whole organism, we perform a genome-wide transgenic RNAi screen through ubiquitous gene knockdowns, focusing on regulators of adult Drosophila testis germline stem cells (GSCs). Here we identify 530 genes that regulate GSC maintenance and differentiation. Of these, we further knock down 113 selected genes using cell-type-specific Gal4s and find that more than half were external regulators, that is, from the local microenvironment or more distal sources. Some genes, for example, versatile (vers), encoding a heterochromatin protein, regulates GSC fates differentially in different cell types and through multiple pathways. We also find that mitosis/cytokinesis proteins are especially important for male GSC maintenance. Our findings provide valuable insights and resources for studying stem cell regulation at the organismal level.

Suggested Citation

  • Ying Liu & Qinglan Ge & Brian Chan & Hanhan Liu & Shree Ram Singh & Jacob Manley & Jae Lee & Ann Marie Weideman & Gerald Hou & Steven X. Hou, 2016. "Whole-animal genome-wide RNAi screen identifies networks regulating male germline stem cells in Drosophila," Nature Communications, Nature, vol. 7(1), pages 1-11, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12149
    DOI: 10.1038/ncomms12149
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