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Global changes of the RNA-bound proteome during the maternal-to-zygotic transition in Drosophila

Author

Listed:
  • Vasiliy O. Sysoev

    (European Molecular Biology Laboratory)

  • Bernd Fischer

    (European Molecular Biology Laboratory
    German Cancer Research Center, Im Neuenheimer Feld 580)

  • Christian K. Frese

    (European Molecular Biology Laboratory)

  • Ishaan Gupta

    (European Molecular Biology Laboratory)

  • Jeroen Krijgsveld

    (European Molecular Biology Laboratory)

  • Matthias W. Hentze

    (European Molecular Biology Laboratory)

  • Alfredo Castello

    (European Molecular Biology Laboratory
    University of Oxford)

  • Anne Ephrussi

    (European Molecular Biology Laboratory)

Abstract

The maternal-to-zygotic transition (MZT) is a process that occurs in animal embryos at the earliest developmental stages, during which maternally deposited mRNAs and other molecules are degraded and replaced by products of the zygotic genome. The zygotic genome is not activated immediately upon fertilization, and in the pre-MZT embryo post-transcriptional control by RNA-binding proteins (RBPs) orchestrates the first steps of development. To identify relevant Drosophila RBPs organism-wide, we refined the RNA interactome capture method for comparative analysis of the pre- and post-MZT embryos. We determine 523 proteins as high-confidence RBPs, half of which were not previously reported to bind RNA. Comparison of the RNA interactomes of pre- and post-MZT embryos reveals high dynamicity of the RNA-bound proteome during early development, and suggests active regulation of RNA binding of some RBPs. This resource provides unprecedented insight into the system of RBPs that govern the earliest steps of Drosophila development.

Suggested Citation

  • Vasiliy O. Sysoev & Bernd Fischer & Christian K. Frese & Ishaan Gupta & Jeroen Krijgsveld & Matthias W. Hentze & Alfredo Castello & Anne Ephrussi, 2016. "Global changes of the RNA-bound proteome during the maternal-to-zygotic transition in Drosophila," Nature Communications, Nature, vol. 7(1), pages 1-11, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12128
    DOI: 10.1038/ncomms12128
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    Cited by:

    1. JohnCarlo Kristofich & Christopher V. Nicchitta, 2023. "Signal-noise metrics for RNA binding protein identification reveal broad spectrum protein-RNA interaction frequencies and dynamics," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    2. Meeli Mullari & Nicolas Fossat & Niels H. Skotte & Andrea Asenjo-Martinez & David T. Humphreys & Jens Bukh & Agnete Kirkeby & Troels K. H. Scheel & Michael L. Nielsen, 2023. "Characterising the RNA-binding protein atlas of the mammalian brain uncovers RBM5 misregulation in mouse models of Huntington’s disease," Nature Communications, Nature, vol. 14(1), pages 1-20, December.

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