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The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

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  • Carsten Rautengarten

    (Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA
    ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne)

  • Berit Ebert

    (Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA
    ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne
    Faculty of Science, University of Copenhagen)

  • Lifeng Liu

    (University of California)

  • Solomon Stonebloom

    (Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA)

  • Andreia M. Smith-Moritz

    (Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA)

  • Markus Pauly

    (University of California)

  • Ariel Orellana

    (Centro de Biotecnología Vegetal, Facultad de Ciencias Biológicas, Universidad Andrés Bello
    Fondo de Areas Prioritarias Center for Genome Regulation)

  • Henrik Vibe Scheller

    (Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA
    University of California)

  • Joshua L. Heazlewood

    (Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA
    ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne)

Abstract

Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

Suggested Citation

  • Carsten Rautengarten & Berit Ebert & Lifeng Liu & Solomon Stonebloom & Andreia M. Smith-Moritz & Markus Pauly & Ariel Orellana & Henrik Vibe Scheller & Joshua L. Heazlewood, 2016. "The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development," Nature Communications, Nature, vol. 7(1), pages 1-10, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12119
    DOI: 10.1038/ncomms12119
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