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The RanBP2/RanGAP1*SUMO1/Ubc9 SUMO E3 ligase is a disassembly machine for Crm1-dependent nuclear export complexes

Author

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  • Tobias Ritterhoff

    (Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance
    University of Washington)

  • Hrishikesh Das

    (Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance
    Cryo Electron Microscopy, CellNetworks, BioQuant, Universitätsklinikum Heidelberg)

  • Götz Hofhaus

    (Cryo Electron Microscopy, CellNetworks, BioQuant, Universitätsklinikum Heidelberg)

  • Rasmus R. Schröder

    (Cryo Electron Microscopy, CellNetworks, BioQuant, Universitätsklinikum Heidelberg)

  • Annette Flotho

    (Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance)

  • Frauke Melchior

    (Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance)

Abstract

Continuous cycles of nucleocytoplasmic transport require disassembly of transport receptor/Ran-GTP complexes in the cytoplasm. A basic disassembly mechanism in all eukaryotes depends on soluble RanGAP and RanBP1. In vertebrates, a significant fraction of RanGAP1 stably interacts with the nucleoporin RanBP2 at a binding site that is flanked by FG-repeats and Ran-binding domains, and overlaps with RanBP2’s SUMO E3 ligase region. Here, we show that the RanBP2/RanGAP1*SUMO1/Ubc9 complex functions as an autonomous disassembly machine with a preference for the export receptor Crm1. We describe three in vitro reconstituted disassembly intermediates, which show binding of a Crm1 export complex via two FG-repeat patches, cargo-release by RanBP2’s Ran-binding domains and retention of free Crm1 at RanBP2 after Ran-GTP hydrolysis. Intriguingly, all intermediates are compatible with SUMO E3 ligase activity, suggesting that the RanBP2/RanGAP1*SUMO1/Ubc9 complex may link Crm1- and SUMO-dependent functions.

Suggested Citation

  • Tobias Ritterhoff & Hrishikesh Das & Götz Hofhaus & Rasmus R. Schröder & Annette Flotho & Frauke Melchior, 2016. "The RanBP2/RanGAP1*SUMO1/Ubc9 SUMO E3 ligase is a disassembly machine for Crm1-dependent nuclear export complexes," Nature Communications, Nature, vol. 7(1), pages 1-13, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11482
    DOI: 10.1038/ncomms11482
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    Cited by:

    1. Xiaomei Zhang & Lihao Ge & Guanghui Jin & Yasong Liu & Qingfen Yu & Weizhao Chen & Liang Chen & Tao Dong & Kiyoharu J. Miyagishima & Juan Shen & Jinghong Yang & Guo Lv & Yan Xu & Qing Yang & Linsen Ye, 2024. "Cold-induced FOXO1 nuclear transport aids cold survival and tissue storage," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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