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Unconventional endosome-like compartment and retromer complex in Toxoplasma gondii govern parasite integrity and host infection

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  • Lamba Omar Sangaré

    (Center for Infection and Immunity of Lille, INSERM U 1019, CNRS UMR 8204, Institut Pasteur de Lille, Université de Lille)

  • Tchilabalo Dilezitoko Alayi

    (Laboratory of Bio-Organic Mass Spectrometry, IPHC, CNRS UMR 7178, Université de Strasbourg
    Plateforme de Protéomique et des Peptides Modifiés (P3M), Institut Pasteur de Lille, CNRS, Université de Lille)

  • Benoit Westermann

    (Laboratory of Bio-Organic Mass Spectrometry, IPHC, CNRS UMR 7178, Université de Strasbourg)

  • Agnes Hovasse

    (Laboratory of Bio-Organic Mass Spectrometry, IPHC, CNRS UMR 7178, Université de Strasbourg)

  • Fabien Sindikubwabo

    (CNRS UMR5163, LAPM, Université Joseph Fourier)

  • Isabelle Callebaut

    (CNRS UMR7590, Sorbonne Universités, Université Pierre et Marie Curie-Paris 6, MNHN, IRD-IUC)

  • Elisabeth Werkmeister

    (Bioimaging Platform, IBL, CNRS, Université de Lille)

  • Frank Lafont

    (Bioimaging Platform, IBL, CNRS, Université de Lille)

  • Christian Slomianny

    (Laboratory of Cell Physiology, INSERM U 1003, Université de Lille)

  • Mohamed-Ali Hakimi

    (CNRS UMR5163, LAPM, Université Joseph Fourier)

  • Alain Van Dorsselaer

    (Laboratory of Bio-Organic Mass Spectrometry, IPHC, CNRS UMR 7178, Université de Strasbourg)

  • Christine Schaeffer-Reiss

    (Laboratory of Bio-Organic Mass Spectrometry, IPHC, CNRS UMR 7178, Université de Strasbourg)

  • Stanislas Tomavo

    (Center for Infection and Immunity of Lille, INSERM U 1019, CNRS UMR 8204, Institut Pasteur de Lille, Université de Lille
    Plateforme de Protéomique et des Peptides Modifiés (P3M), Institut Pasteur de Lille, CNRS, Université de Lille)

Abstract

Membrane trafficking pathways play critical roles in Apicomplexa, a phylum of protozoan parasites that cause life-threatening diseases worldwide. Here we report the first retromer-trafficking interactome in Toxoplasma gondii. This retromer complex includes a trimer Vps35–Vps26–Vps29 core complex that serves as a hub for the endosome-like compartment and parasite-specific proteins. Conditional ablation of TgVps35 reveals that the retromer complex is crucial for the biogenesis of secretory organelles and for maintaining parasite morphology. We identify TgHP12 as a parasite-specific and retromer-associated protein with functions unrelated to secretory organelle formation. Furthermore, the major facilitator superfamily homologue named TgHP03, which is a multiple spanning and ligand transmembrane transporter, is maintained at the parasite membrane by retromer-mediated endocytic recycling. Thus, our findings highlight that both evolutionarily conserved and unconventional proteins act in concert in T. gondii by controlling retrograde transport that is essential for parasite integrity and host infection.

Suggested Citation

  • Lamba Omar Sangaré & Tchilabalo Dilezitoko Alayi & Benoit Westermann & Agnes Hovasse & Fabien Sindikubwabo & Isabelle Callebaut & Elisabeth Werkmeister & Frank Lafont & Christian Slomianny & Mohamed-A, 2016. "Unconventional endosome-like compartment and retromer complex in Toxoplasma gondii govern parasite integrity and host infection," Nature Communications, Nature, vol. 7(1), pages 1-14, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11191
    DOI: 10.1038/ncomms11191
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