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The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis

Author

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  • Zhicheng Shao

    (Stem Cell Institute, University of Alabama at Birmingham)

  • Ruowen Zhang

    (Stem Cell Institute, University of Alabama at Birmingham)

  • Alireza Khodadadi-Jamayran

    (Stem Cell Institute, University of Alabama at Birmingham)

  • Bo Chen

    (Stem Cell Institute, University of Alabama at Birmingham)

  • Michael R. Crowley

    (Howell and Elizabeth Heflin Center for Genomic Science, University of Alabama at Birmingham)

  • Muhamad A. Festok

    (Stem Cell Institute, University of Alabama at Birmingham)

  • David K. Crossman

    (Howell and Elizabeth Heflin Center for Genomic Science, University of Alabama at Birmingham)

  • Tim M. Townes

    (Stem Cell Institute, University of Alabama at Birmingham)

  • Kejin Hu

    (Stem Cell Institute, University of Alabama at Birmingham)

Abstract

It is well known that both recipient cells and donor nuclei demonstrate a mitotic advantage as observed in the traditional reprogramming with somatic cell nuclear transfer (SCNT). However, it is not known whether a specific mitotic factor plays a critical role in reprogramming. Here we identify an isoform of human bromodomain-containing 3 (BRD3), BRD3R (BRD3 with Reprogramming activity), as a reprogramming factor. BRD3R positively regulates mitosis during reprogramming, upregulates a large set of mitotic genes at early stages of reprogramming, and associates with mitotic chromatin. Interestingly, a set of the mitotic genes upregulated by BRD3R constitutes a pluripotent molecular signature. The two BRD3 isoforms display differential binding to acetylated histones. Our results suggest a molecular interpretation for the mitotic advantage in reprogramming and show that mitosis may be a driving force of reprogramming.

Suggested Citation

  • Zhicheng Shao & Ruowen Zhang & Alireza Khodadadi-Jamayran & Bo Chen & Michael R. Crowley & Muhamad A. Festok & David K. Crossman & Tim M. Townes & Kejin Hu, 2016. "The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis," Nature Communications, Nature, vol. 7(1), pages 1-15, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10869
    DOI: 10.1038/ncomms10869
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