IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms10423.html
   My bibliography  Save this article

Membrane potential shapes regulation of dopamine transporter trafficking at the plasma membrane

Author

Listed:
  • Ben D. Richardson

    (Evelyn F. and William L. McKnight Brain Institute, University of Florida)

  • Kaustuv Saha

    (Evelyn F. and William L. McKnight Brain Institute, University of Florida)

  • Danielle Krout

    (University of North Dakota School of Medicine and Health Sciences)

  • Elizabeth Cabrera

    (Evelyn F. and William L. McKnight Brain Institute, University of Florida)

  • Bruce Felts

    (University of North Dakota School of Medicine and Health Sciences)

  • L. Keith Henry

    (University of North Dakota School of Medicine and Health Sciences)

  • Jarod Swant

    (Evelyn F. and William L. McKnight Brain Institute, University of Florida)

  • Mu-Fa Zou

    (Medicinal Chemistry Section, Intramural Research Program, National Institute on Drug Abuse)

  • Amy Hauck Newman

    (Medicinal Chemistry Section, Intramural Research Program, National Institute on Drug Abuse)

  • Habibeh Khoshbouei

    (Evelyn F. and William L. McKnight Brain Institute, University of Florida)

Abstract

The dopaminergic system is essential for cognitive processes, including reward, attention and motor control. In addition to DA release and availability of synaptic DA receptors, timing and magnitude of DA neurotransmission depend on extracellular DA-level regulation by the dopamine transporter (DAT), the membrane expression and trafficking of which are highly dynamic. Data presented here from real-time TIRF (TIRFM) and confocal microscopy coupled with surface biotinylation and electrophysiology suggest that changes in the membrane potential alone, a universal yet dynamic cellular property, rapidly alter trafficking of DAT to and from the surface membrane. Broadly, these findings suggest that cell-surface DAT levels are sensitive to membrane potential changes, which can rapidly drive DAT internalization from and insertion into the cell membrane, thus having an impact on the capacity for DAT to regulate extracellular DA levels.

Suggested Citation

  • Ben D. Richardson & Kaustuv Saha & Danielle Krout & Elizabeth Cabrera & Bruce Felts & L. Keith Henry & Jarod Swant & Mu-Fa Zou & Amy Hauck Newman & Habibeh Khoshbouei, 2016. "Membrane potential shapes regulation of dopamine transporter trafficking at the plasma membrane," Nature Communications, Nature, vol. 7(1), pages 1-12, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10423
    DOI: 10.1038/ncomms10423
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms10423
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms10423?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10423. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.