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Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores

Author

Listed:
  • Janko Kajtez

    (Max Planck Institute of Molecular Cell Biology and Genetics)

  • Anastasia Solomatina

    (Max Planck Institute of Molecular Cell Biology and Genetics)

  • Maja Novak

    (Faculty of Science, University of Zagreb)

  • Bruno Polak

    (Max Planck Institute of Molecular Cell Biology and Genetics
    Ruđer Bošković Institute)

  • Kruno Vukušić

    (Ruđer Bošković Institute)

  • Jonas Rüdiger

    (Max Planck Institute of Molecular Cell Biology and Genetics)

  • Gheorghe Cojoc

    (Max Planck Institute of Molecular Cell Biology and Genetics)

  • Ana Milas

    (Ruđer Bošković Institute)

  • Ivana Šumanovac Šestak

    (Max Planck Institute of Molecular Cell Biology and Genetics
    Ruđer Bošković Institute)

  • Patrik Risteski

    (Ruđer Bošković Institute)

  • Federica Tavano

    (Max Planck Institute of Molecular Cell Biology and Genetics)

  • Anna H. Klemm

    (Max Planck Institute of Molecular Cell Biology and Genetics)

  • Emanuele Roscioli

    (Virginia Tech
    Virginia Bioinformatics Institute, Virginia Tech
    Present address: Mechanochemical Cell Biology Building, Division of Biomedical Cell Biology, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK.)

  • Julie Welburn

    (Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Michael Swann Building, Max Born Crescent)

  • Daniela Cimini

    (Virginia Tech
    Virginia Bioinformatics Institute, Virginia Tech)

  • Matko Glunčić

    (Faculty of Science, University of Zagreb)

  • Nenad Pavin

    (Faculty of Science, University of Zagreb)

  • Iva M. Tolić

    (Max Planck Institute of Molecular Cell Biology and Genetics
    Ruđer Bošković Institute)

Abstract

During metaphase, forces on kinetochores are exerted by k-fibres, bundles of microtubules that end at the kinetochore. Interestingly, non-kinetochore microtubules have been observed between sister kinetochores, but their function is unknown. Here we show by laser-cutting of a k-fibre in HeLa and PtK1 cells that a bundle of non-kinetochore microtubules, which we term ‘bridging fibre’, bridges sister k-fibres and balances the interkinetochore tension. We found PRC1 and EB3 in the bridging fibre, suggesting that it consists of antiparallel dynamic microtubules. By using a theoretical model that includes a bridging fibre, we show that the forces at the pole and at the kinetochore depend on the bridging fibre thickness. Moreover, our theory and experiments show larger relaxation of the interkinetochore distance for cuts closer to kinetochores. We conclude that the bridging fibre, by linking sister k-fibres, withstands the tension between sister kinetochores and enables the spindle to obtain a curved shape.

Suggested Citation

  • Janko Kajtez & Anastasia Solomatina & Maja Novak & Bruno Polak & Kruno Vukušić & Jonas Rüdiger & Gheorghe Cojoc & Ana Milas & Ivana Šumanovac Šestak & Patrik Risteski & Federica Tavano & Anna H. Klemm, 2016. "Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores," Nature Communications, Nature, vol. 7(1), pages 1-11, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10298
    DOI: 10.1038/ncomms10298
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    Cited by:

    1. Isabella Koprivec & Valentina Štimac & Mario Đura & Kruno Vukušić & Petra Mikec & Iva M. Tolić, 2026. "Polar chromosomes are rescued from missegregation by spindle elongation-driven microtubule pivoting," Nature Communications, Nature, vol. 17(1), pages 1-21, December.
    2. Kruno Vukušić & Iva M. Tolić, 2025. "CENP-E initiates chromosome congression by opposing Aurora kinases to promote end-on attachments," Nature Communications, Nature, vol. 16(1), pages 1-19, December.
    3. Dalileh Nabi & Hauke Drechsler & Johannes Pschirer & Franz Korn & Nadine Schuler & Stefan Diez & Rolf Jessberger & Mariola Chacón, 2021. "CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    4. Wei Ming Lim & Wei-Xiang Chew & Arianna Esposito Verza & Marion Pesenti & Andrea Musacchio & Thomas Surrey, 2024. "Regulation of minimal spindle midzone organization by mitotic kinases," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    5. Ai Kiyomitsu & Toshiya Nishimura & Shiang Jyi Hwang & Satoshi Ansai & Masato T. Kanemaki & Minoru Tanaka & Tomomi Kiyomitsu, 2024. "Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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