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F-actin mechanics control spindle centring in the mouse zygote

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  • Agathe Chaigne

    (CIRB, Collège de France, and CNRS-UMR7241 and INSERM-U1050, Equipe labellisée Ligue contre le Cancer
    Present address: Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT, UK.)

  • Clément Campillo

    (Université Evry Val d’Essonne, LAMBE, Boulevard F Mitterrand)

  • Raphaël Voituriez

    (UMR7600-CNRS/UPMC)

  • Nir S. Gov

    (Weizmann Institute of Science)

  • Cécile Sykes

    (CNRS-UMR168
    UPMC
    Institut Curie, Centre de Recherche, Laboratoire Physico-Chimie)

  • Marie-Hélène Verlhac

    (CIRB, Collège de France, and CNRS-UMR7241 and INSERM-U1050, Equipe labellisée Ligue contre le Cancer)

  • Marie-Emilie Terret

    (CIRB, Collège de France, and CNRS-UMR7241 and INSERM-U1050, Equipe labellisée Ligue contre le Cancer)

Abstract

Mitotic spindle position relies on interactions between astral microtubules nucleated by centrosomes and a rigid cortex. Some cells, such as mouse oocytes, do not possess centrosomes and astral microtubules. These cells rely only on actin and on a soft cortex to position their spindle off-centre and undergo asymmetric divisions. While the first mouse embryonic division also occurs in the absence of centrosomes, it is symmetric and not much is known on how the spindle is positioned at the exact cell centre. Using interdisciplinary approaches, we demonstrate that zygotic spindle positioning follows a three-step process: (1) coarse centring of pronuclei relying on the dynamics of an F-actin/Myosin-Vb meshwork; (2) fine centring of the metaphase plate depending on a high cortical tension; (3) passive maintenance at the cell centre. Altogether, we show that F-actin-dependent mechanics operate the switch between asymmetric to symmetric division required at the oocyte to embryo transition.

Suggested Citation

  • Agathe Chaigne & Clément Campillo & Raphaël Voituriez & Nir S. Gov & Cécile Sykes & Marie-Hélène Verlhac & Marie-Emilie Terret, 2016. "F-actin mechanics control spindle centring in the mouse zygote," Nature Communications, Nature, vol. 7(1), pages 1-14, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10253
    DOI: 10.1038/ncomms10253
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