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Spindle assembly checkpoint inactivation fails to suppress neuroblast tumour formation in aurA mutant Drosophila

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  • Renaud Caous

    (Institut de Génétique et Développement de Rennes-Université de Rennes I-CNRS- UMR 6290)

  • Aude Pascal

    (Institut de Génétique et Développement de Rennes-Université de Rennes I-CNRS- UMR 6290)

  • Pierre Romé

    (Institut de Génétique et Développement de Rennes-Université de Rennes I-CNRS- UMR 6290
    Present address: The Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Michael Swann Building, Max Born Crescent, Edinburgh EH9 3BF, UK)

  • Laurent Richard-Parpaillon

    (Institut de Génétique et Développement de Rennes-Université de Rennes I-CNRS- UMR 6290)

  • Roger Karess

    (Institut Jacques Monod-Université Paris Diderot-Paris 7)

  • Régis Giet

    (Institut de Génétique et Développement de Rennes-Université de Rennes I-CNRS- UMR 6290)

Abstract

Tissue homeostasis requires accurate control of cell proliferation, differentiation and chromosome segregation. Drosophila sas-4 and aurA mutants present brain tumours with extra neuroblasts (NBs), defective mitotic spindle assembly and delayed mitosis due to activation of the spindle assembly checkpoint (SAC). Here we inactivate the SAC in aurA and sas-4 mutants to determine whether the generation of aneuploidy compromises NB proliferation. Inactivation of the SAC in the sas-4 mutant impairs NB proliferation and disrupts euploidy. By contrast, disrupting the SAC in the aurA mutant does not prevent NB amplification, tumour formation or chromosome segregation. The monitoring of Mad2 and cyclin B dynamics in live aurA NBs reveals that SAC satisfaction is not coupled to cyclin B degradation. Thus, the NBs of aurA mutants present delayed mitosis, with accurate chromosome segregation occurring in a SAC-independent manner. We report here the existence of an Aurora A-dependent mechanism promoting efficient, timed cyclin B degradation.

Suggested Citation

  • Renaud Caous & Aude Pascal & Pierre Romé & Laurent Richard-Parpaillon & Roger Karess & Régis Giet, 2015. "Spindle assembly checkpoint inactivation fails to suppress neuroblast tumour formation in aurA mutant Drosophila," Nature Communications, Nature, vol. 6(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9879
    DOI: 10.1038/ncomms9879
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