IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms9192.html
   My bibliography  Save this article

mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3

Author

Listed:
  • Anna Gebhardt

    (Innate Immunity Laboratory, Max-Planck Institute of Biochemistry)

  • Matthias Habjan

    (Innate Immunity Laboratory, Max-Planck Institute of Biochemistry)

  • Christian Benda

    (Max-Planck Institute of Biochemistry)

  • Arno Meiler

    (Innate Immunity Laboratory, Max-Planck Institute of Biochemistry)

  • Darya A. Haas

    (Innate Immunity Laboratory, Max-Planck Institute of Biochemistry)

  • Marco Y. Hein

    (Max-Planck Institute of Biochemistry)

  • Angelika Mann

    (Innate Immunity Laboratory, Max-Planck Institute of Biochemistry)

  • Matthias Mann

    (Max-Planck Institute of Biochemistry)

  • Bianca Habermann

    (Bioinformatics Core Facility, Max-Planck Institute of Biochemistry)

  • Andreas Pichlmair

    (Innate Immunity Laboratory, Max-Planck Institute of Biochemistry)

Abstract

The flow of genetic information from DNA to protein requires polymerase-II-transcribed RNA characterized by the presence of a 5′-cap. The cap-binding complex (CBC), consisting of the nuclear cap-binding protein (NCBP) 2 and its adaptor NCBP1, is believed to bind all capped RNA and to be necessary for its processing and intracellular localization. Here we show that NCBP1, but not NCBP2, is required for cell viability and poly(A) RNA export. We identify C17orf85 (here named NCBP3) as a cap-binding protein that together with NCBP1 forms an alternative CBC in higher eukaryotes. NCBP3 binds mRNA, associates with components of the mRNA processing machinery and contributes to poly(A) RNA export. Loss of NCBP3 can be compensated by NCBP2 under steady-state conditions. However, NCBP3 becomes pivotal under stress conditions, such as virus infection. We propose the existence of an alternative CBC involving NCBP1 and NCBP3 that plays a key role in mRNA biogenesis.

Suggested Citation

  • Anna Gebhardt & Matthias Habjan & Christian Benda & Arno Meiler & Darya A. Haas & Marco Y. Hein & Angelika Mann & Matthias Mann & Bianca Habermann & Andreas Pichlmair, 2015. "mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3," Nature Communications, Nature, vol. 6(1), pages 1-13, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9192
    DOI: 10.1038/ncomms9192
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms9192
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms9192?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9192. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.