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An Arabidopsis PWI and RRM motif-containing protein is critical for pre-mRNA splicing and ABA responses

Author

Listed:
  • Xiangqiang Zhan

    (Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences)

  • Bilian Qian

    (University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA)

  • Fengqiu Cao

    (Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences)

  • Wenwu Wu

    (Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences)

  • Lan Yang

    (Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences)

  • Qingmei Guan

    (University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA)

  • Xianbin Gu

    (University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA)

  • Pengcheng Wang

    (Purdue University)

  • Temiloluwa A. Okusolubo

    (University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA)

  • Stephanie L. Dunn

    (University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA)

  • Jian-Kang Zhu

    (Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences
    Purdue University)

  • Jianhua Zhu

    (University of Maryland, 2121 Plant Sciences Building, College Park, Maryland 20742, USA)

Abstract

The phytohormone abscisic acid (ABA) is important for growth, development and stress responses in plants. Recent research has identified ABA receptors and signalling components that regulate seed germination and stomatal closure. However, proteins that regulate ABA signalling remain poorly understood. Here we use a forward-genetic screen to identify rbm25-1 and rbm25-2, two Arabidopsis mutants with increased sensitivity to growth inhibition by ABA. Using RNA-seq, we found that RBM25 controls the splicing of many pre-mRNAs. The protein phosphatase 2C HAB1, a critical component in ABA signalling, shows a dramatic defect in pre-mRNA splicing in rbm25 mutants. Ectopic expression of a HAB1 complementary DNA derived from wild-type mRNAs partially suppresses the rbm25-2 mutant phenotype. We suggest that RNA splicing is of particular importance for plant response to ABA and that the splicing factor RBM25 has a critical role in this response.

Suggested Citation

  • Xiangqiang Zhan & Bilian Qian & Fengqiu Cao & Wenwu Wu & Lan Yang & Qingmei Guan & Xianbin Gu & Pengcheng Wang & Temiloluwa A. Okusolubo & Stephanie L. Dunn & Jian-Kang Zhu & Jianhua Zhu, 2015. "An Arabidopsis PWI and RRM motif-containing protein is critical for pre-mRNA splicing and ABA responses," Nature Communications, Nature, vol. 6(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9139
    DOI: 10.1038/ncomms9139
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