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Cell shape dynamics during the staphylococcal cell cycle

Author

Listed:
  • João M. Monteiro

    (Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa)

  • Pedro B. Fernandes

    (Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa)

  • Filipa Vaz

    (Laboratory of Bacterial Cell Surfaces and Pathogenesis, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa)

  • Ana R. Pereira

    (Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa)

  • Andreia C. Tavares

    (Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa)

  • Maria T. Ferreira

    (Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa)

  • Pedro M. Pereira

    (Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa)

  • Helena Veiga

    (Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa)

  • Erkin Kuru

    (Indiana University Bloomington
    Indiana University Bloomington)

  • Michael S. VanNieuwenhze

    (Indiana University Bloomington)

  • Yves V. Brun

    (Indiana University Bloomington)

  • Sérgio R. Filipe

    (Laboratory of Bacterial Cell Surfaces and Pathogenesis, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa)

  • Mariana G. Pinho

    (Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa)

Abstract

Staphylococcus aureus is an aggressive pathogen and a model organism to study cell division in sequential orthogonal planes in spherical bacteria. However, the small size of staphylococcal cells has impaired analysis of changes in morphology during the cell cycle. Here we use super-resolution microscopy and determine that S. aureus cells are not spherical throughout the cell cycle, but elongate during specific time windows, through peptidoglycan synthesis and remodelling. Both peptidoglycan hydrolysis and turgor pressure are required during division for reshaping the flat division septum into a curved surface. In this process, the septum generates less than one hemisphere of each daughter cell, a trait we show is common to other cocci. Therefore, cell surface scars of previous divisions do not divide the cells in quadrants, generating asymmetry in the daughter cells. Our results introduce a need to reassess the models for division plane selection in cocci.

Suggested Citation

  • João M. Monteiro & Pedro B. Fernandes & Filipa Vaz & Ana R. Pereira & Andreia C. Tavares & Maria T. Ferreira & Pedro M. Pereira & Helena Veiga & Erkin Kuru & Michael S. VanNieuwenhze & Yves V. Brun & , 2015. "Cell shape dynamics during the staphylococcal cell cycle," Nature Communications, Nature, vol. 6(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9055
    DOI: 10.1038/ncomms9055
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