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Light generation of intracellular Ca2+ signals by a genetically encoded protein BACCS

Author

Listed:
  • Tomohiro Ishii

    (Graduate School of Medical and Dental Science, Tokyo Medical and Dental University
    The Center for Brain Integration Research, Tokyo Medical and Dental University)

  • Koji Sato

    (Okazaki Institute for Integrative Bioscience
    Institute of Industrial Science, The University of Tokyo
    ERATO Takeuchi Biohybrid Innovation Project, Japan Science and Technology Agency
    Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Toshiyuki Kakumoto

    (Graduate School of Medical and Dental Science, Tokyo Medical and Dental University
    The Center for Brain Integration Research, Tokyo Medical and Dental University)

  • Shigenori Miura

    (Institute of Industrial Science, The University of Tokyo
    ERATO Takeuchi Biohybrid Innovation Project, Japan Science and Technology Agency)

  • Kazushige Touhara

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo
    ERATO Touhara Chemosensory Signal Project, JST, The University of Tokyo)

  • Shoji Takeuchi

    (Institute of Industrial Science, The University of Tokyo
    ERATO Takeuchi Biohybrid Innovation Project, Japan Science and Technology Agency)

  • Takao Nakata

    (Graduate School of Medical and Dental Science, Tokyo Medical and Dental University
    The Center for Brain Integration Research, Tokyo Medical and Dental University)

Abstract

Ca2+ signals are highly regulated in a spatiotemporal manner in numerous cellular physiological events. Here we report a genetically engineered blue light-activated Ca2+ channel switch (BACCS), as an optogenetic tool for generating Ca2+ signals. BACCS opens Ca2+-selective ORAI ion channels in response to light. A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca2+ concentration more rapidly, such that Ca2+ elevation in mammalian cells is observed within 1 s on light exposure. Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression. In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses. Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca2+. Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca2+ signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.

Suggested Citation

  • Tomohiro Ishii & Koji Sato & Toshiyuki Kakumoto & Shigenori Miura & Kazushige Touhara & Shoji Takeuchi & Takao Nakata, 2015. "Light generation of intracellular Ca2+ signals by a genetically encoded protein BACCS," Nature Communications, Nature, vol. 6(1), pages 1-15, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9021
    DOI: 10.1038/ncomms9021
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