IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms8978.html
   My bibliography  Save this article

Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation

Author

Listed:
  • Lukas Lercher

    (University of Oxford, Chemistry Research Laboratory
    Present address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany)

  • Ritu Raj

    (University of Oxford, Chemistry Research Laboratory)

  • Nisha A. Patel

    (University of Oxford, Physical and Theoretical Chemistry Laboratory)

  • Joshua Price

    (University of Oxford, Chemistry Research Laboratory)

  • Shabaz Mohammed

    (University of Oxford, Chemistry Research Laboratory)

  • Carol V. Robinson

    (University of Oxford, Physical and Theoretical Chemistry Laboratory)

  • Christopher J. Schofield

    (University of Oxford, Chemistry Research Laboratory)

  • Benjamin G. Davis

    (University of Oxford, Chemistry Research Laboratory)

Abstract

O-GlcNAcylation is a newly discovered histone modification implicated in transcriptional regulation, but no structural information on the physical effect of GlcNAcylation on chromatin exists. Here, we generate synthetic, pure GlcNAcylated histones and nucleosomes and reveal that GlcNAcylation can modulate structure through direct destabilization of H2A/H2B dimers in the nucleosome, thus promoting an ‘open’ chromatin state. The results suggest that a plausible molecular basis for one role of histone O-GlcNAcylation in epigenetic regulation is to lower the barrier for RNA polymerase passage and hence increase transcription.

Suggested Citation

  • Lukas Lercher & Ritu Raj & Nisha A. Patel & Joshua Price & Shabaz Mohammed & Carol V. Robinson & Christopher J. Schofield & Benjamin G. Davis, 2015. "Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation," Nature Communications, Nature, vol. 6(1), pages 1-9, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8978
    DOI: 10.1038/ncomms8978
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms8978
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms8978?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8978. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.