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The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores

Author

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  • Ho-Soo Lee

    (Ajou University School of Medicine
    Graduate School of Ajou University)

  • Yong-Yea Park

    (Ajou University School of Medicine)

  • Mi-Young Cho

    (Ajou University School of Medicine
    Graduate School of Ajou University)

  • Sunyoung Chae

    (Ajou University School of Medicine)

  • Young-Suk Yoo

    (Ajou University School of Medicine
    Graduate School of Ajou University)

  • Myung-Hee Kwon

    (Graduate School of Ajou University
    Ajou University School of Medicine)

  • Chang-Woo Lee

    (Sungkyunkwan University School of Medicine)

  • Hyeseong Cho

    (Ajou University School of Medicine
    Graduate School of Ajou University)

Abstract

Accumulation of PLK1 at kinetochores is essential for chromosome alignment and segregation; however, the mechanism underlying PLK1 recruitment to kinetochores remains unresolved. The chromatin remodeller RSF1 tightly associates with centromere proteins, but its mitotic function is unknown. Here we show that RSF1 localizes at mitotic kinetochores and directly binds PLK1. RSF1 depletion disrupts localization of PLK1 at kinetochores; the C-terminal fragment of RSF1, which can bind PLK1, is sufficient to restore PLK1 localization. Moreover, CDK1 phosphorylates RSF1 at Ser1375, and this phosphorylation is necessary for PLK1 recruitment. Subsequently, PLK1 phosphorylates RSF1 at Ser1359, stabilizing PLK1 deposition. Importantly, RSF1 depletion mimicks the chromosome misalignment phenotype resulting from PLK1 knockdown; these defects are rescued by RSF1 S1375D or RSF1 S1359D but not RSF1 S1375A, showing a functional link between phosphorylation of RSF1 and chromosome alignment. Together, these data show that RSF1 is an essential centromeric component that recruits PLK1 to kinetochores and plays a crucial role in faithful cell division.

Suggested Citation

  • Ho-Soo Lee & Yong-Yea Park & Mi-Young Cho & Sunyoung Chae & Young-Suk Yoo & Myung-Hee Kwon & Chang-Woo Lee & Hyeseong Cho, 2015. "The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores," Nature Communications, Nature, vol. 6(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8904
    DOI: 10.1038/ncomms8904
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    Cited by:

    1. Ho-Soo Lee & Sunwoo Min & Ye-Eun Jung & Sunyoung Chae & June Heo & Jae-Ho Lee & TaeSoo Kim & Ho-Chul Kang & Makoto Nakanish & Sun-Shin Cha & Hyeseong Cho, 2021. "Spatiotemporal coordination of the RSF1-PLK1-Aurora B cascade establishes mitotic signaling platforms," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Amit Frishberg & Neta Milman & Ayelet Alpert & Hannah Spitzer & Ben Asani & Johannes B. Schiefelbein & Evgeny Bakin & Karen Regev-Berman & Siegfried G. Priglinger & Joachim L. Schultze & Fabian J. The, 2023. "Reconstructing disease dynamics for mechanistic insights and clinical benefit," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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