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An actin-dependent spindle position checkpoint ensures the asymmetric division in mouse oocytes

Author

Listed:
  • Aïcha Metchat

    (European Molecular Biology Laboratory)

  • Manuel Eguren

    (European Molecular Biology Laboratory)

  • Julius M. Hossain

    (European Molecular Biology Laboratory)

  • Antonio Z. Politi

    (European Molecular Biology Laboratory)

  • Sébastien Huet

    (European Molecular Biology Laboratory)

  • Jan Ellenberg

    (European Molecular Biology Laboratory)

Abstract

Faithful chromosome segregation, during meiosis, is of critical importance to prevent aneuploidy in the resulting embryo. In mammalian oocytes, the segregation of homologous chromosomes takes place with the spindle located at the cell’s periphery. The spindle is often assembled close to the centre of the cell, which necessitates the actin network for spindle transport to the cell cortex. In this study, we investigate how the segregation of chromosomes is coordinated with the positioning of the metaphase I spindle. We develop different assays to perturb the spindle’s position and to delay its relocation to the cell periphery. We find that anaphase is delayed until the spindle is positioned in close proximity with the oocyte cortex. We further show that the metaphase arrest is dependent on a functional actin network, in addition to the spindle assembly checkpoint. Our work provides the first evidence for the existence of a functional spindle position checkpoint.

Suggested Citation

  • Aïcha Metchat & Manuel Eguren & Julius M. Hossain & Antonio Z. Politi & Sébastien Huet & Jan Ellenberg, 2015. "An actin-dependent spindle position checkpoint ensures the asymmetric division in mouse oocytes," Nature Communications, Nature, vol. 6(1), pages 1-10, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8784
    DOI: 10.1038/ncomms8784
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