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Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR

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  • Yoon Seok Suh

    (Neurophysiology Research Group, Bio-Nano Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yusong-gu, Daejeon 305-806, Korea
    Korea University of Science and Technology (UST))

  • Shreelatha Bhat

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Seung-Hyun Hong

    (Neurophysiology Research Group, Bio-Nano Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yusong-gu, Daejeon 305-806, Korea)

  • Minjung Shin

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Suhyoung Bahk

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Kyung Sang Cho

    (Konkuk University)

  • Seung-Whan Kim

    (Chungnam National University Hospital)

  • Kyu-Sun Lee

    (Neurophysiology Research Group, Bio-Nano Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yusong-gu, Daejeon 305-806, Korea
    Korea University of Science and Technology (UST))

  • Young-Joon Kim

    (School of Life Sciences, Gwangju Institute of Science and Technology (GIST))

  • Walton D. Jones

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Kweon Yu

    (Neurophysiology Research Group, Bio-Nano Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yusong-gu, Daejeon 305-806, Korea
    Korea University of Science and Technology (UST))

Abstract

MicroRNAs (miRNAs) regulate many physiological processes including body growth. Insulin/IGF signalling is the primary regulator of animal body growth, but the extent to which miRNAs act in insulin-producing cells (IPCs) is unclear. Here we generate a UAS-miRNA library of Drosophila stocks and perform a genetic screen to identify miRNAs whose overexpression in the IPCs inhibits body growth in Drosophila. Through this screen, we identify miR-9a as an evolutionarily conserved regulator of insulin signalling and body growth. IPC-specific miR-9a overexpression reduces insulin signalling and body size. Of the predicted targets of miR-9a, we find that loss of miR-9a enhances the level of sNPFR1. We show via an in vitro binding assay that miR-9a binds to sNPFR1 mRNA in insect cells and to the mammalian orthologue NPY2R in rat insulinoma cells. These findings indicate that the conserved miR-9a regulates body growth by controlling sNPFR1/NPYR-mediated modulation of insulin signalling.

Suggested Citation

  • Yoon Seok Suh & Shreelatha Bhat & Seung-Hyun Hong & Minjung Shin & Suhyoung Bahk & Kyung Sang Cho & Seung-Whan Kim & Kyu-Sun Lee & Young-Joon Kim & Walton D. Jones & Kweon Yu, 2015. "Genome-wide microRNA screening reveals that the evolutionary conserved miR-9a regulates body growth by targeting sNPFR1/NPYR," Nature Communications, Nature, vol. 6(1), pages 1-11, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8693
    DOI: 10.1038/ncomms8693
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