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Rh D blood group conversion using transcription activator-like effector nucleases

Author

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  • Young-Hoon Kim

    (Graduate School of Biomedical Science and Engineering/College of Medicine, Hanyang University
    Brain Korea 21 Plus Project for Medical Sciences, Graduate Program of Nano Science and Technology, Yonsei University College of Medicine)

  • Hyun O. Kim

    (Yonsei University College of Medicine)

  • Eun J. Baek

    (Hanyang University College of Medicine)

  • Ryo Kurita

    (Central Blood Institute, Japanese Red Cross Society)

  • Hyuk-Jin Cha

    (College of Natural Sciences, Sogang University)

  • Yukio Nakamura

    (RIKEN BioResource Center)

  • Hyongbum Kim

    (Graduate School of Biomedical Science and Engineering/College of Medicine, Hanyang University
    Brain Korea 21 Plus Project for Medical Sciences, Graduate Program of Nano Science and Technology, Yonsei University College of Medicine)

Abstract

Group O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a 12-transmembrane domain protein. Here we convert Rh D-positive erythroid progenitor cells into D-negative cells using RHD-targeting transcription activator-like effector nucleases (TALENs). After transfection of TALEN-encoding plasmids, RHD-knockout clones are obtained. Erythroid-lineage cells differentiated from these knockout erythroid progenitor cells do not agglutinate in the presence of anti-D reagents and do not express D antigen, as assessed using flow cytometry. Our programmable nuclease-induced blood group conversion opens new avenues for compatible donor cell generation in transfusion medicine.

Suggested Citation

  • Young-Hoon Kim & Hyun O. Kim & Eun J. Baek & Ryo Kurita & Hyuk-Jin Cha & Yukio Nakamura & Hyongbum Kim, 2015. "Rh D blood group conversion using transcription activator-like effector nucleases," Nature Communications, Nature, vol. 6(1), pages 1-13, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8451
    DOI: 10.1038/ncomms8451
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