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Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours

Author

Listed:
  • Tan A. Ince

    (Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami)

  • Aurea D. Sousa

    (Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami)

  • Michelle A. Jones

    (Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami)

  • J. Chuck Harrell

    (Lineberger Comprehensive Cancer Center, University of North Carolina)

  • Elin S. Agoston

    (Brigham and Women’s Hospital, Harvard Medical School)

  • Marit Krohn

    (MD Anderson Cancer Center)

  • Laura M. Selfors

    (Harvard Medical School)

  • Wenbin Liu

    (MD Anderson Cancer Center)

  • Ken Chen

    (MD Anderson Cancer Center)

  • Mao Yong

    (MD Anderson Cancer Center)

  • Peter Buchwald

    (University of Miami Miller School of Medicine)

  • Bin Wang

    (Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami)

  • Katherine S. Hale

    (MD Anderson Cancer Center)

  • Evan Cohick

    (Brigham and Women’s Hospital, Harvard Medical School)

  • Petra Sergent

    (Vincent Center for Reproductive Biology, Massachusetts General Hospital and Harvard Medical School)

  • Abigail Witt

    (Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami)

  • Zhanna Kozhekbaeva

    (Interdisciplinary Stem Cell Institute, Braman Family Breast Cancer Institute, and Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami)

  • Sizhen Gao

    (Harvard Medical School)

  • Agoston T. Agoston

    (Brigham and Women’s Hospital, Harvard Medical School)

  • Melissa A. Merritt

    (Brigham and Women’s Hospital, Harvard Medical School)

  • Rosemary Foster

    (Vincent Center for Reproductive Biology, Massachusetts General Hospital and Harvard Medical School)

  • Bo R. Rueda

    (Vincent Center for Reproductive Biology, Massachusetts General Hospital and Harvard Medical School)

  • Christopher P. Crum

    (Brigham and Women’s Hospital, Harvard Medical School)

  • Joan S. Brugge

    (Harvard Medical School)

  • Gordon B. Mills

    (MD Anderson Cancer Center)

Abstract

Currently available human tumour cell line panels consist of a small number of lines in each lineage that generally fail to retain the phenotype of the original patient tumour. Here we develop a cell culture medium that enables us to routinely establish cell lines from diverse subtypes of human ovarian cancers with >95% efficiency. Importantly, the 25 new ovarian tumour cell lines described here retain the genomic landscape, histopathology and molecular features of the original tumours. Furthermore, the molecular profile and drug response of these cell lines correlate with distinct groups of primary tumours with different outcomes. Thus, tumour cell lines derived using this methodology represent a significantly improved platform to study human tumour pathophysiology and response to therapy.

Suggested Citation

  • Tan A. Ince & Aurea D. Sousa & Michelle A. Jones & J. Chuck Harrell & Elin S. Agoston & Marit Krohn & Laura M. Selfors & Wenbin Liu & Ken Chen & Mao Yong & Peter Buchwald & Bin Wang & Katherine S. Hal, 2015. "Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours," Nature Communications, Nature, vol. 6(1), pages 1-14, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8419
    DOI: 10.1038/ncomms8419
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