IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms8287.html
   My bibliography  Save this article

Vimentin filament organization and stress sensing depend on its single cysteine residue and zinc binding

Author

Listed:
  • Dolores Pérez-Sala

    (Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (C.S.I.C.))

  • Clara L. Oeste

    (Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (C.S.I.C.))

  • Alma E. Martínez

    (Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (C.S.I.C.))

  • M. Jesús Carrasco

    (Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (C.S.I.C.))

  • Beatriz Garzón

    (Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (C.S.I.C.))

  • F. Javier Cañada

    (Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (C.S.I.C.))

Abstract

The vimentin filament network plays a key role in cell architecture and signalling, as well as in epithelial–mesenchymal transition. Vimentin C328 is targeted by various oxidative modifications, but its role in vimentin organization is not known. Here we show that C328 is essential for vimentin network reorganization in response to oxidants and electrophiles, and is required for optimal vimentin performance in network expansion, lysosomal distribution and aggresome formation. C328 may fulfil these roles through interaction with zinc. In vitro, micromolar zinc protects vimentin from iodoacetamide modification and elicits vimentin polymerization into optically detectable structures; in cells, zinc closely associates with vimentin and its depletion causes reversible filament disassembly. Finally, zinc transport-deficient human fibroblasts show increased vimentin solubility and susceptibility to disruption, which are restored by zinc supplementation. These results unveil a critical role of C328 in vimentin organization and open new perspectives for the regulation of intermediate filaments by zinc.

Suggested Citation

  • Dolores Pérez-Sala & Clara L. Oeste & Alma E. Martínez & M. Jesús Carrasco & Beatriz Garzón & F. Javier Cañada, 2015. "Vimentin filament organization and stress sensing depend on its single cysteine residue and zinc binding," Nature Communications, Nature, vol. 6(1), pages 1-17, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8287
    DOI: 10.1038/ncomms8287
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms8287
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms8287?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8287. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.