Author
Listed:
- Satoshi Fukuyama
(Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Institute of Medical Science, University of Tokyo)
- Hiroaki Katsura
(Institute of Medical Science, University of Tokyo)
- Dongming Zhao
(Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Institute of Medical Science, University of Tokyo)
- Makoto Ozawa
(Laboratory of Animal Hygiene, Joint Faculty of Veterinary Medicine, Kagoshima University
Transboundary Animal Distance Center, Joint Faculty of Veterinary Medicine, Kagoshima University)
- Tomomi Ando
(Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Institute of Medical Science, University of Tokyo)
- Jason E. Shoemaker
(Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Institute of Medical Science, University of Tokyo)
- Izumi Ishikawa
(Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Institute of Medical Science, University of Tokyo)
- Shinya Yamada
(Institute of Medical Science, University of Tokyo)
- Gabriele Neumann
(School of Veterinary Medicine, University of Wisconsin-Madison)
- Shinji Watanabe
(Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Laboratory of Veterinary Microbiology, University of Miyazaki)
- Hiroaki Kitano
(Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
The Systems Biology Institute
Sony Computer Science Laboratories
Okinawa Institute of Science and Technology Graduate University)
- Yoshihiro Kawaoka
(Exploratory Research for Advanced Technology Infection-Induced Host Responses Project, Japan Science and Technology Agency (JST)
Institute of Medical Science, University of Tokyo
School of Veterinary Medicine, University of Wisconsin-Madison
International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo)
Abstract
Seasonal influenza A viruses cause annual epidemics of respiratory disease; highly pathogenic avian H5N1 and the recently emerged H7N9 viruses cause severe infections in humans, often with fatal outcomes. Although numerous studies have addressed the pathogenicity of influenza viruses, influenza pathogenesis remains incompletely understood. Here we generate influenza viruses expressing fluorescent proteins of different colours (‘Color-flu’ viruses) to facilitate the study of viral infection in in vivo models. On adaptation to mice, stable expression of the fluorescent proteins in infected animals allows their detection by different types of microscopy and by flow cytometry. We use this system to analyse the progression of viral spread in mouse lungs, for live imaging of virus-infected cells, and for differential gene expression studies in virus antigen-positive and virus antigen-negative live cells in the lungs of Color-flu-infected mice. Collectively, Color-flu viruses are powerful tools to analyse virus infections at the cellular level in vivo to better understand influenza pathogenesis.
Suggested Citation
Satoshi Fukuyama & Hiroaki Katsura & Dongming Zhao & Makoto Ozawa & Tomomi Ando & Jason E. Shoemaker & Izumi Ishikawa & Shinya Yamada & Gabriele Neumann & Shinji Watanabe & Hiroaki Kitano & Yoshihiro , 2015.
"Multi-spectral fluorescent reporter influenza viruses (Color-flu) as powerful tools for in vivo studies,"
Nature Communications, Nature, vol. 6(1), pages 1-8, May.
Handle:
RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7600
DOI: 10.1038/ncomms7600
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