IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v6y2015i1d10.1038_ncomms7535.html
   My bibliography  Save this article

The class II PI 3-kinase, PI3KC2α, links platelet internal membrane structure to shear-dependent adhesive function

Author

Listed:
  • Jessica K. Mountford

    (Australian Centre for Blood Diseases, Monash University)

  • Claire Petitjean

    (Australian Centre for Blood Diseases, Monash University)

  • Harun W. Kusuma Putra

    (Australian Centre for Blood Diseases, Monash University)

  • Jonathan A. McCafferty

    (Australian Centre for Blood Diseases, Monash University)

  • Natasha M. Setiabakti

    (Australian Centre for Blood Diseases, Monash University)

  • Hannah Lee

    (Australian Centre for Blood Diseases, Monash University)

  • Lotte L. Tønnesen

    (Australian Centre for Blood Diseases, Monash University)

  • James D. McFadyen

    (Australian Centre for Blood Diseases, Monash University)

  • Simone M. Schoenwaelder

    (Australian Centre for Blood Diseases, Monash University
    The Heart Research Institute and Charles Perkins Centre, The University of Sydney)

  • Anita Eckly

    (Unité mixte de recherche S949 Institut National de la Santé et de la Recherche Médicale, Université de Strasbourg, Etablissement Français du Sang-Alsace 67000)

  • Christian Gachet

    (Unité mixte de recherche S949 Institut National de la Santé et de la Recherche Médicale, Université de Strasbourg, Etablissement Français du Sang-Alsace 67000)

  • Sarah Ellis

    (Peter MacCallum Cancer Centre and The University of Melbourne)

  • Anne K. Voss

    (Walter and Eliza Hall Institute of Medical Research
    University of Melbourne)

  • Ross A. Dickins

    (Walter and Eliza Hall Institute of Medical Research
    University of Melbourne)

  • Justin R. Hamilton

    (Australian Centre for Blood Diseases, Monash University)

  • Shaun P. Jackson

    (Australian Centre for Blood Diseases, Monash University
    The Heart Research Institute and Charles Perkins Centre, The University of Sydney
    The Scripps Research Institute)

Abstract

PI3KC2α is a broadly expressed lipid kinase with critical functions during embryonic development but poorly defined roles in adult physiology. Here we utilize multiple mouse genetic models to uncover a role for PI3KC2α in regulating the internal membrane reserve structure of megakaryocytes (demarcation membrane system) and platelets (open canalicular system) that results in dysregulated platelet adhesion under haemodynamic shear stress. Structural alterations in the platelet internal membrane lead to enhanced membrane tether formation that is associated with accelerated, yet highly unstable, thrombus formation in vitro and in vivo. Notably, agonist-induced 3-phosphorylated phosphoinositide production and cellular activation are normal in PI3KC2α-deficient platelets. These findings demonstrate an important role for PI3KC2α in regulating shear-dependent platelet adhesion via regulation of membrane structure, rather than acute signalling. These studies provide a link between the open canalicular system and platelet adhesive function that has relevance to the primary haemostatic and prothrombotic function of platelets.

Suggested Citation

  • Jessica K. Mountford & Claire Petitjean & Harun W. Kusuma Putra & Jonathan A. McCafferty & Natasha M. Setiabakti & Hannah Lee & Lotte L. Tønnesen & James D. McFadyen & Simone M. Schoenwaelder & Anita , 2015. "The class II PI 3-kinase, PI3KC2α, links platelet internal membrane structure to shear-dependent adhesive function," Nature Communications, Nature, vol. 6(1), pages 1-14, May.
  • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7535
    DOI: 10.1038/ncomms7535
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms7535
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/ncomms7535?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7535. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.