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Local endocytosis triggers dendritic thinning and pruning in Drosophila sensory neurons

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  • Takahiro Kanamori

    (Graduate School of Science, The University of Tokyo
    Osaka Bioscience Institute
    Present address: Biozentrum, University of Basel, Klingelbergstrasse 50/70, Basel 4056, Switzerland)

  • Jiro Yoshino

    (Graduate School of Science, The University of Tokyo)

  • Kei-ichiro Yasunaga

    (Graduate School of Science, The University of Tokyo
    Osaka Bioscience Institute)

  • Yusuke Dairyo

    (Graduate School of Science, The University of Tokyo
    Osaka Bioscience Institute)

  • Kazuo Emoto

    (Graduate School of Science, The University of Tokyo
    Osaka Bioscience Institute)

Abstract

The refinement of neural circuits involves dendrite pruning, a process that removes inappropriate projections that are formed during development. In Drosophila sensory neurons, compartmentalized calcium (Ca2+) transients in dendrites act as spatiotemporal cues to trigger pruning, yet how neurons define the dendrites with Ca2+ transients remains elusive. Here we report that local elevation of endocytic activity contributes to defining dendrites that generate Ca2+ transients, triggering pruning. In vivo imaging of single dendrites reveals an increase of endocytosis in proximal dendrites that spatially and temporally correlates with dendrite thinning, an early step in pruning tightly coupled with compartmentalized Ca2+ transients. Two GTPases, Rab5 and dynamin, are required for both the increased endocytic activity and compartmentalized Ca2+ transients. Further genetic analyses suggest that local endocytosis in proximal dendrites functions cooperatively with global endocytosis-mediated protein degradation pathways to promote dendrite pruning.

Suggested Citation

  • Takahiro Kanamori & Jiro Yoshino & Kei-ichiro Yasunaga & Yusuke Dairyo & Kazuo Emoto, 2015. "Local endocytosis triggers dendritic thinning and pruning in Drosophila sensory neurons," Nature Communications, Nature, vol. 6(1), pages 1-14, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7515
    DOI: 10.1038/ncomms7515
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    Cited by:

    1. Mami Nakamizo-Dojo & Kenichi Ishii & Jiro Yoshino & Masato Tsuji & Kazuo Emoto, 2023. "Descending GABAergic pathway links brain sugar-sensing to peripheral nociceptive gating in Drosophila," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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