Author
Listed:
- Nitzan Zandany
(Zlotowski Center for Neurosciences, Ben-Gurion University of the Negev)
- Shir Marciano
(Zlotowski Center for Neurosciences, Ben-Gurion University of the Negev)
- Elhanan Magidovich
(Zlotowski Center for Neurosciences, Ben-Gurion University of the Negev)
- Teddy Frimerman
(Zlotowski Center for Neurosciences, Ben-Gurion University of the Negev)
- Rinat Yehezkel
(Zlotowski Center for Neurosciences, Ben-Gurion University of the Negev)
- Tzilhav Shem-Ad
(Zlotowski Center for Neurosciences, Ben-Gurion University of the Negev)
- Limor Lewin
(Zlotowski Center for Neurosciences, Ben-Gurion University of the Negev)
- Uri Abdu
(Zlotowski Center for Neurosciences, Ben-Gurion University of the Negev)
- Irit Orr
(Zlotowski Center for Neurosciences, Ben-Gurion University of the Negev)
- Ofer Yifrach
(Zlotowski Center for Neurosciences, Ben-Gurion University of the Negev)
Abstract
Ion channel clustering at the post-synaptic density serves a fundamental role in action potential generation and transmission. Here, we show that interaction between the Shaker Kv channel and the PSD-95 scaffold protein underlying channel clustering is modulated by the length of the intrinsically disordered C terminal channel tail. We further show that this tail functions as an entropic clock that times PSD-95 binding. We thus propose a ‘ball and chain’ mechanism to explain Kv channel binding to scaffold proteins, analogous to the mechanism describing channel fast inactivation. The physiological relevance of this mechanism is demonstrated in that alternative splicing of the Shaker channel gene to produce variants of distinct tail lengths resulted in differential channel cell surface expression levels and clustering metrics that correlate with differences in affinity of the variants for PSD-95. We suggest that modulating channel clustering by specific spatial-temporal spliced variant targeting serves a fundamental role in nervous system development and tuning.
Suggested Citation
Nitzan Zandany & Shir Marciano & Elhanan Magidovich & Teddy Frimerman & Rinat Yehezkel & Tzilhav Shem-Ad & Limor Lewin & Uri Abdu & Irit Orr & Ofer Yifrach, 2015.
"Alternative splicing modulates Kv channel clustering through a molecular ball and chain mechanism,"
Nature Communications, Nature, vol. 6(1), pages 1-12, May.
Handle:
RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7488
DOI: 10.1038/ncomms7488
Download full text from publisher
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7488. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.