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A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6+CD27− subset

Author

Listed:
  • Elham Kashani

    (Institute of Immunology, Hannover Medical School)

  • Lisa Föhse

    (Institute of Immunology, Hannover Medical School)

  • Solaiman Raha

    (Institute of Immunology, Hannover Medical School)

  • Inga Sandrock

    (Institute of Immunology, Hannover Medical School)

  • Linda Oberdörfer

    (Institute of Immunology, Hannover Medical School)

  • Christian Koenecke

    (Institute of Immunology, Hannover Medical School
    Haemostasis, Oncology and Stem-Cell Transplantation, Hannover Medical School)

  • Sebastian Suerbaum

    (Institute of Medical Microbiology and Hospital Epidemiology, Hannover Medical School)

  • Siegfried Weiss

    (Helmholtz Centre for Infection Research)

  • Immo Prinz

    (Institute of Immunology, Hannover Medical School)

Abstract

Here we investigate the TCR repertoire of mouse Vγ4+ γδ T cells in correlation with their developmental origin and homeostasis. By deep sequencing we identify a high frequency of straight Vδ5Dδ2Jδ1 germline rearrangements without P- and N-nucleotides within the otherwise highly diverse Trd repertoire of Vγ4+ cells. This sequence is infrequent in CCR6−CD27+ cells, but abundant among CCR6+CD27− γδ T cells. Using an inducible Rag1 knock-in mouse model, we show that γδ T cells generated in the adult thymus rarely contain this germline-rearranged Vδ5Dδ2Jδ1 sequence, confirming its fetal origin. Single-cell analysis and deep sequencing of the Trg locus reveal a dominant CDR3 junctional motif that completes the TCR repertoire of invariant Vγ4+Vδ5+ cells. In conclusion, this study identifies an innate subset of fetal thymus-derived γδ T cells with an invariant Vγ4+Vδ5+ TCR that is restricted to the CCR6+CD27− subset of γδ T cells.

Suggested Citation

  • Elham Kashani & Lisa Föhse & Solaiman Raha & Inga Sandrock & Linda Oberdörfer & Christian Koenecke & Sebastian Suerbaum & Siegfried Weiss & Immo Prinz, 2015. "A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6+CD27− subset," Nature Communications, Nature, vol. 6(1), pages 1-11, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7477
    DOI: 10.1038/ncomms7477
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