Author
Listed:
- Chiara Bonfanti
(University of Milan)
- Giuliana Rossi
(University of Milan)
- Francesco Saverio Tedesco
(University College London)
- Monica Giannotta
(IFOM, FIRC Institute of Molecular Oncology)
- Sara Benedetti
(University College London
Institute of Child Health, University College London)
- Rossana Tonlorenzi
(San Raffaele Hospital)
- Stefania Antonini
(University of Milan)
- Giovanna Marazzi
(Stem Cells and Regenerative Medicine, ICAN UMRS 1166 Inserm/Sorbonne University (UPMC ParisVI))
- Elisabetta Dejana
(University of Milan
IFOM, FIRC Institute of Molecular Oncology)
- David Sassoon
(Stem Cells and Regenerative Medicine, ICAN UMRS 1166 Inserm/Sorbonne University (UPMC ParisVI))
- Giulio Cossu
(University of Milan
University College London
Institute of Inflammation and Repair, University of Manchester)
- Graziella Messina
(University of Milan)
Abstract
Mesoangioblasts are vessel-associated progenitor cells that show therapeutic promise for the treatment of muscular dystrophy. Mesoangioblasts have the ability to undergo skeletal muscle differentiation and cross the blood vessel wall regardless of the developmental stage at which they are isolated. Here we show that PW1/Peg3 is expressed at high levels in mesoangioblasts obtained from mouse, dog and human tissues and its level of expression correlates with their myogenic competence. Silencing PW1/Peg3 markedly inhibits myogenic potential of mesoangioblasts in vitro through MyoD degradation. Moreover, lack of PW1/Peg3 abrogates mesoangioblast ability to cross the vessel wall and to engraft into damaged myofibres through the modulation of the junctional adhesion molecule-A. We conclude that PW1/Peg3 function is essential for conferring proper mesoangioblast competence and that the determination of PW1/Peg3 levels in human mesoangioblasts may serve as a biomarker to identify the best donor populations for therapeutic application in muscular dystrophies.
Suggested Citation
Chiara Bonfanti & Giuliana Rossi & Francesco Saverio Tedesco & Monica Giannotta & Sara Benedetti & Rossana Tonlorenzi & Stefania Antonini & Giovanna Marazzi & Elisabetta Dejana & David Sassoon & Giuli, 2015.
"PW1/Peg3 expression regulates key properties that determine mesoangioblast stem cell competence,"
Nature Communications, Nature, vol. 6(1), pages 1-13, May.
Handle:
RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7364
DOI: 10.1038/ncomms7364
Download full text from publisher
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7364. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.