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Host genetic determinants of microbiota-dependent nutrition revealed by genome-wide analysis of Drosophila melanogaster

Author

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  • Adam J. Dobson

    (Department of Entomology
    Present address: Department of Genetics, Evolution and Environment, Institute of Healthy Ageing, University College London, Gower Street, London WC1E 6BT, UK)

  • John M. Chaston

    (Department of Entomology
    Present address: Department of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah 84602, USA)

  • Peter D. Newell

    (Department of Entomology
    Present address: Biological Sciences, SUNY Oswego, Oswego, New York 13126-3599, USA)

  • Leanne Donahue

    (Department of Entomology)

  • Sara L. Hermann

    (Department of Entomology)

  • David R. Sannino

    (Cornell University)

  • Stephanie Westmiller

    (Department of Entomology)

  • Adam C.-N. Wong

    (Department of Entomology
    Present address: School of Biological Sciences, University of Sydney, Sydney, New South Wales 2006, Australia)

  • Andrew G. Clark

    (Cornell University)

  • Brian P. Lazzaro

    (Department of Entomology)

  • Angela E. Douglas

    (Department of Entomology
    Cornell University)

Abstract

Animals bear communities of gut microorganisms with substantial effects on animal nutrition, but the host genetic basis of these effects is unknown. Here we use Drosophila to demonstrate substantial among-genotype variation in the effects of eliminating the gut microbiota on five host nutritional indices (weight, protein, lipid, glucose and glycogen contents); this includes variation in both the magnitude and direction of microbiota-dependent effects. Genome-wide association studies to identify the genetic basis of the microbiota-dependent variation reveal polymorphisms in largely non-overlapping sets of genes associated with variation in the nutritional traits, including strong representation of conserved genes functioning in signalling. Key genes identified by the GWA study are validated by loss-of-function mutations that altered microbiota-dependent nutritional effects. We conclude that the microbiota interacts with the animal at multiple points in the signalling and regulatory networks that determine animal nutrition. These interactions with the microbiota are probably conserved across animals, including humans.

Suggested Citation

  • Adam J. Dobson & John M. Chaston & Peter D. Newell & Leanne Donahue & Sara L. Hermann & David R. Sannino & Stephanie Westmiller & Adam C.-N. Wong & Andrew G. Clark & Brian P. Lazzaro & Angela E. Dougl, 2015. "Host genetic determinants of microbiota-dependent nutrition revealed by genome-wide analysis of Drosophila melanogaster," Nature Communications, Nature, vol. 6(1), pages 1-9, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7312
    DOI: 10.1038/ncomms7312
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