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A rapid bioluminescence assay for measuring myeloperoxidase activity in human plasma

Author

Listed:
  • Reece J. Goiffon

    (Mallinckrodt Institute of Radiology, Washington University School of Medicine)

  • Sara C. Martinez

    (Mallinckrodt Institute of Radiology, Washington University School of Medicine)

  • David Piwnica-Worms

    (Mallinckrodt Institute of Radiology, Washington University School of Medicine
    University of Texas MD Anderson Cancer Center, 1400 Pressler Street, Unit 1479, FCT16.6030, Houston, Texas 77030, USA)

Abstract

Myeloperoxidase (MPO) is a circulating cardiovascular disease (CVD) biomarker used to estimate clinical risk and patient prognosis. Current enzyme-linked immunosorbent assays (ELISA) for MPO concentration are costly and time-intensive. Here we report a novel bioluminescence assay, designated MPO activity on a polymer surface (MAPS), for measuring MPO activity in human plasma samples using the bioluminescent substrate L-012. The method delivers a result in under an hour and is resistant to confounding effects from endogenous MPO inhibitors. In a pilot clinical study, we compared MAPS and two clinical ELISAs using 72 plasma samples from cardiac catheterization patients. Results from parallel MAPS and ELISAs were concordant within 2±11 μg l−1 MPO with similar uncertainty and reproducibility. Results between parallel MAPS and ELISA were in better agreement than those between independent ELISAs. MAPS may provide an inexpensive and rapid assay for determining MPO activity in plasma samples from patients with CVD or potentially other immune and inflammatory disorders.

Suggested Citation

  • Reece J. Goiffon & Sara C. Martinez & David Piwnica-Worms, 2015. "A rapid bioluminescence assay for measuring myeloperoxidase activity in human plasma," Nature Communications, Nature, vol. 6(1), pages 1-9, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7271
    DOI: 10.1038/ncomms7271
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