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Primitive macrophages control HSPC mobilization and definitive haematopoiesis

Author

Listed:
  • Jana Travnickova

    (CNRS UMR 5235
    Universite Montpellier 2, Dynamique des Interactions Membranaires Normales et Pathologiques
    Universite Montpellier 1)

  • Vanessa Tran Chau

    (CNRS UMR 5235
    Universite Montpellier 2, Dynamique des Interactions Membranaires Normales et Pathologiques
    Universite Montpellier 1
    Institut Pasteur, Unité Macrophages et Développement de l'Immunité)

  • Emmanuelle Julien

    (INSERM UMR_S949
    Universite de Strasbourg
    Etablissement Français du Sang-Alsace)

  • Julio Mateos-Langerak

    (Montpellier RIO Imaging)

  • Catherine Gonzalez

    (CNRS UMR 5235
    Universite Montpellier 2, Dynamique des Interactions Membranaires Normales et Pathologiques
    Universite Montpellier 1)

  • Etienne Lelièvre

    (CNRS UMR 5235
    Universite Montpellier 2, Dynamique des Interactions Membranaires Normales et Pathologiques
    Universite Montpellier 1
    INSERM, DIMNP)

  • Georges Lutfalla

    (CNRS UMR 5235
    Universite Montpellier 2, Dynamique des Interactions Membranaires Normales et Pathologiques
    Universite Montpellier 1)

  • Manuela Tavian

    (INSERM UMR_S949
    Universite de Strasbourg
    Etablissement Français du Sang-Alsace)

  • Karima Kissa

    (CNRS UMR 5235
    Universite Montpellier 2, Dynamique des Interactions Membranaires Normales et Pathologiques
    Universite Montpellier 1
    INSERM, DIMNP)

Abstract

In vertebrates, haematopoietic stem/progenitor cells (HSPCs) first emerge in the aorta–gonad–mesonephros (AGM) before colonizing transitory and subsequently definitive haematopoietic organs allowing haematopoiesis throughout adult life. Here we identify an unexpected primitive macrophage population accumulated in the dorsal mesenteric mesoderm surrounding the dorsal aorta of the human embryo and study its function in the transparent zebrafish embryo. Our study reveals dynamic interactions occurring between the HSPCs and primitive macrophages in the AGM. Specific chemical and inducible genetic depletion of macrophages or inhibition of matrix metalloproteinases (Mmps) leads to an accumulation of HSPCs in the AGM and a decrease in the colonization of haematopoietic organs. Finally, in vivo zymography demonstrates the function of primitive macrophages in extracellular matrix degradation, which allows HSPC migration through the AGM stroma, their intravasation, leading to the colonization of haematopoietic organs and the establishment of definitive haematopoiesis.

Suggested Citation

  • Jana Travnickova & Vanessa Tran Chau & Emmanuelle Julien & Julio Mateos-Langerak & Catherine Gonzalez & Etienne Lelièvre & Georges Lutfalla & Manuela Tavian & Karima Kissa, 2015. "Primitive macrophages control HSPC mobilization and definitive haematopoiesis," Nature Communications, Nature, vol. 6(1), pages 1-9, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7227
    DOI: 10.1038/ncomms7227
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    Cited by:

    1. Xiaoyi Cheng & Radwa Barakat & Giulia Pavani & Masuma Khatun Usha & Rodolfo Calderon & Elizabeth Snella & Abigail Gorden & Yudi Zhang & Paul Gadue & Deborah L. French & Karin S. Dorman & Antonella Fid, 2023. "Nod1-dependent NF-kB activation initiates hematopoietic stem cell specification in response to small Rho GTPases," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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