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Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR–eIF4F pathway

Author

Listed:
  • Andrej Susor

    (Institute of Animal Physiology and Genetics, ASCR)

  • Denisa Jansova

    (Institute of Animal Physiology and Genetics, ASCR)

  • Renata Cerna

    (Institute of Animal Physiology and Genetics, ASCR)

  • Anna Danylevska

    (CEITEC-Veterinary Research Institute)

  • Martin Anger

    (Institute of Animal Physiology and Genetics, ASCR
    CEITEC-Veterinary Research Institute)

  • Tereza Toralova

    (Institute of Animal Physiology and Genetics, ASCR)

  • Radek Malik

    (Institute of Molecular Genetics, ASCR)

  • Jaroslava Supolikova

    (Institute of Animal Physiology and Genetics, ASCR)

  • Matthew S. Cook

    (The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, UCSF)

  • Jeong Su Oh

    (Sungkyunkwan University)

  • Michal Kubelka

    (Institute of Animal Physiology and Genetics, ASCR)

Abstract

The fully grown mammalian oocyte is transcriptionally quiescent and utilizes only transcripts synthesized and stored during early development. However, we find that an abundant RNA population is retained in the oocyte nucleus and contains specific mRNAs important for meiotic progression. Here we show that during the first meiotic division, shortly after nuclear envelope breakdown, translational hotspots develop in the chromosomal area and in a region that was previously surrounded the nucleus. These distinct translational hotspots are separated by endoplasmic reticulum and Lamin, and disappear following polar body extrusion. Chromosomal translational hotspots are controlled by the activity of the mTOR–eIF4F pathway. Here we reveal a mechanism that—following the resumption of meiosis—controls the temporal and spatial translation of a specific set of transcripts required for normal spindle assembly, chromosome alignment and segregation.

Suggested Citation

  • Andrej Susor & Denisa Jansova & Renata Cerna & Anna Danylevska & Martin Anger & Tereza Toralova & Radek Malik & Jaroslava Supolikova & Matthew S. Cook & Jeong Su Oh & Michal Kubelka, 2015. "Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR–eIF4F pathway," Nature Communications, Nature, vol. 6(1), pages 1-12, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7078
    DOI: 10.1038/ncomms7078
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