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A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform

Author

Listed:
  • Howon Lee

    (Institutes of Entrepreneurial BioConvergence, Seoul National University)

  • Hyoki Kim

    (Celemics Inc., 371-17, Gasan-dong, Geumcheon-gu)

  • Sungsik Kim

    (Institutes of Entrepreneurial BioConvergence, Seoul National University
    Interdisciplinary Program for Bioengineering, Seoul National University)

  • Taehoon Ryu

    (Institutes of Entrepreneurial BioConvergence, Seoul National University
    Seoul National University)

  • Hwangbeom Kim

    (University of California, Los Angeles)

  • Duhee Bang

    (Yonsei University)

  • Sunghoon Kwon

    (Institutes of Entrepreneurial BioConvergence, Seoul National University
    Seoul National University
    Center for Nanoparticle Research, Institute for Basic Science, Seoul National University
    Seoul National University Hospital Biomedical Research Institute 101 Daehakro, Chungrogu, Seoul National University Hospital)

Abstract

Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called ‘Sniper Cloning’ that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.

Suggested Citation

  • Howon Lee & Hyoki Kim & Sungsik Kim & Taehoon Ryu & Hwangbeom Kim & Duhee Bang & Sunghoon Kwon, 2015. "A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform," Nature Communications, Nature, vol. 6(1), pages 1-7, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7073
    DOI: 10.1038/ncomms7073
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