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A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants

Author

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  • Antoine Larrieu

    (Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon
    Centre for Plant Integrative Biology, University of Nottingham)

  • Antony Champion

    (Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes
    Laboratoire mixte international Adaptation des Plantes et microorganismes associés aux Stress Environnementaux)

  • Jonathan Legrand

    (Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon)

  • Julien Lavenus

    (Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes)

  • David Mast

    (Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon)

  • Géraldine Brunoud

    (Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon)

  • Jaesung Oh

    (Centre for Plant Integrative Biology, University of Nottingham
    Present address: Plasma Technology Research Center, National Fusion Research Institute, Gunsan-City, Republic of Korea)

  • Soazig Guyomarc’h

    (Université Montpellier 2, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes)

  • Maxime Pizot

    (Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes)

  • Edward E. Farmer

    (Université de Lausanne)

  • Colin Turnbull

    (Imperial College London)

  • Teva Vernoux

    (Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon)

  • Malcolm J. Bennett

    (Centre for Plant Integrative Biology, University of Nottingham)

  • Laurent Laplaze

    (Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes
    Laboratoire mixte international Adaptation des Plantes et microorganismes associés aux Stress Environnementaux)

Abstract

Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.

Suggested Citation

  • Antoine Larrieu & Antony Champion & Jonathan Legrand & Julien Lavenus & David Mast & Géraldine Brunoud & Jaesung Oh & Soazig Guyomarc’h & Maxime Pizot & Edward E. Farmer & Colin Turnbull & Teva Vernou, 2015. "A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants," Nature Communications, Nature, vol. 6(1), pages 1-9, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7043
    DOI: 10.1038/ncomms7043
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