Author
Listed:
- Burak Dura
(Research Laboratory of Electronics, 50 Vassar Street, Massachusetts Institute of Technology (MIT)
Massachusetts Institute of Technology (MIT))
- Stephanie K. Dougan
(Whitehead Institute for Biomedical Research, Nine Cambridge Center)
- Marta Barisa
(Whitehead Institute for Biomedical Research, Nine Cambridge Center)
- Melanie M. Hoehl
(Research Laboratory of Electronics, 50 Vassar Street, Massachusetts Institute of Technology (MIT)
Health Sciences and Technology, Massachusetts Institute of Technology (MIT))
- Catherine T. Lo
(Research Laboratory of Electronics, 50 Vassar Street, Massachusetts Institute of Technology (MIT))
- Hidde L. Ploegh
(Whitehead Institute for Biomedical Research, Nine Cambridge Center)
- Joel Voldman
(Research Laboratory of Electronics, 50 Vassar Street, Massachusetts Institute of Technology (MIT)
Massachusetts Institute of Technology (MIT)
Microsystems Technology Laboratory, Massachusetts Institute of Technology (MIT))
Abstract
Establishing a successful immune response requires cell–cell interactions, where the nature of antigen presentation dictates functional outcomes. Methods to study these interactions, however, suffer from limited throughput and a lack of control over cell pairing. Here we describe a microfluidic platform that achieves high-throughput deterministic pairing of lymphocytes with a defined contact time, thereby allowing accurate assessment of early activation events for each pair in controlled microenvironments. More importantly, the platform allows the capture of dynamic processes and static parameters from both partners simultaneously, thus enabling pairwise-correlated multiparametric profiling of lymphocyte interactions over hundreds of pairs in a single experiment. Using our platform, we characterized early activation dynamics of CD8 T cells (OT-1 and TRP1 transnuclear (TN)) and investigated the extent of heterogeneity in T-cell activation and the correlation of multiple readouts. The results establish our platform as a promising tool for quantitative investigation of lymphocyte interactions.
Suggested Citation
Burak Dura & Stephanie K. Dougan & Marta Barisa & Melanie M. Hoehl & Catherine T. Lo & Hidde L. Ploegh & Joel Voldman, 2015.
"Profiling lymphocyte interactions at the single-cell level by microfluidic cell pairing,"
Nature Communications, Nature, vol. 6(1), pages 1-13, May.
Handle:
RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms6940
DOI: 10.1038/ncomms6940
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