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Ultrafast cooling reveals microsecond-scale biomolecular dynamics

Author

Listed:
  • Mark E. Polinkovsky

    (University of California, San Diego
    Present address: Stemedica Cell Technologies, Inc., San Diego, California, USA)

  • Yann Gambin

    (The Scripps Research Institute, La Jolla, California 92037, USA
    Present address: Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia)

  • Priya R. Banerjee

    (The Scripps Research Institute, La Jolla, California 92037, USA)

  • Michael J. Erickstad

    (University of California, San Diego)

  • Alex Groisman

    (University of California, San Diego)

  • Ashok A. Deniz

    (The Scripps Research Institute, La Jolla, California 92037, USA)

Abstract

The temperature-jump technique, in which the sample is rapidly heated by a powerful laser pulse, has been widely used to probe the fast dynamics of folding of proteins and nucleic acids. However, the existing temperature-jump setups tend to involve sophisticated and expensive instrumentation, while providing only modest temperature changes of ~10–15 °C, and the temperature changes are only rapid for heating, but not cooling. Here we present a setup comprising a thermally conductive sapphire substrate with light-absorptive nano-coating, a microfluidic device and a rapidly switched moderate-power infrared laser with the laser beam focused on the nano-coating, enabling heating and cooling of aqueous solutions by ~50 °C on a 1-μs time scale. The setup is used to probe folding and unfolding dynamics of DNA hairpins after direct and inverse temperature jumps, revealing low-pass filter behaviour during periodic temperature variations.

Suggested Citation

  • Mark E. Polinkovsky & Yann Gambin & Priya R. Banerjee & Michael J. Erickstad & Alex Groisman & Ashok A. Deniz, 2014. "Ultrafast cooling reveals microsecond-scale biomolecular dynamics," Nature Communications, Nature, vol. 5(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6737
    DOI: 10.1038/ncomms6737
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