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Programmed folding of DNA origami structures through single-molecule force control

Author

Listed:
  • Wooli Bae

    (National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST
    KAIST
    Present address: Department of Physics and Center for Nanoscience (CeNS), Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, 80539 München, Germany)

  • Kipom Kim

    (National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST
    KAIST)

  • Duyoung Min

    (National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST
    KAIST)

  • Je-Kyung Ryu

    (National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST
    KAIST)

  • Changbong Hyeon

    (Korea Institute for Advanced Study)

  • Tae-Young Yoon

    (National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST
    KAIST)

Abstract

Despite the recent development in the design of DNA origami, its folding yet relies on thermal or chemical annealing methods. We here demonstrate mechanical folding of the DNA origami structure via a pathway that has not been accessible to thermal annealing. Using magnetic tweezers, we stretch a single scaffold DNA with mechanical tension to remove its secondary structures, followed by base pairing of the stretched DNA with staple strands. When the force is subsequently quenched, folding of the DNA nanostructure is completed through displacement between the bound staple strands. Each process in the mechanical folding is well defined and free from kinetic traps, enabling us to complete folding within 10 min. We also demonstrate parallel folding of DNA nanostructures through multiplexed manipulation of the scaffold DNAs. Our results suggest a path towards programmability of the folding pathway of DNA nanostructures.

Suggested Citation

  • Wooli Bae & Kipom Kim & Duyoung Min & Je-Kyung Ryu & Changbong Hyeon & Tae-Young Yoon, 2014. "Programmed folding of DNA origami structures through single-molecule force control," Nature Communications, Nature, vol. 5(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6654
    DOI: 10.1038/ncomms6654
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