Author
Listed:
- Swantje Liedmann
(Institute of Molecular Virology (IMV), Center for Molecular Biology of Inflammation (ZMBE), University of Muenster)
- Eike R. Hrincius
(St. Jude Children’s Research Hospital)
- Cliff Guy
(St. Jude Children’s Research Hospital)
- Darisuren Anhlan
(Institute of Molecular Virology (IMV), Center for Molecular Biology of Inflammation (ZMBE), University of Muenster)
- Rüdiger Dierkes
(Institute of Molecular Virology (IMV), Center for Molecular Biology of Inflammation (ZMBE), University of Muenster)
- Robert Carter
(St. Jude Children’s Research Hospital)
- Gang Wu
(St. Jude Children’s Research Hospital)
- Peter Staeheli
(Institute of Virology, University Medical Center Freiburg)
- Douglas R. Green
(St. Jude Children’s Research Hospital)
- Thorsten Wolff
(Division of Influenza and Other Respiratory Viruses)
- Jonathan A. McCullers
(St. Jude Children’s Research Hospital
University of Tennessee Health Sciences Center)
- Stephan Ludwig
(Institute of Molecular Virology (IMV), Center for Molecular Biology of Inflammation (ZMBE), University of Muenster
Cluster of Excellence Cells in Motion, University of Muenster)
- Christina Ehrhardt
(Institute of Molecular Virology (IMV), Center for Molecular Biology of Inflammation (ZMBE), University of Muenster
Cluster of Excellence Cells in Motion, University of Muenster)
Abstract
The type I interferon (IFN) response represents the first line of defence to invading pathogens. Internalized viral ribonucleoproteins (vRNPs) of negative-strand RNA viruses induce an early IFN response by interacting with retinoic acid inducible gene I (RIG-I) and its recruitment to mitochondria. Here we employ three-dimensional stochastic optical reconstruction microscopy (STORM) to visualize incoming influenza A virus (IAV) vRNPs as helical-like structures associated with mitochondria. Unexpectedly, an early IFN induction in response to vRNPs is not detected. A distinct amino-acid motif in the viral polymerases, PB1/PA, suppresses early IFN induction. Mutation of this motif leads to reduced pathogenicity in vivo, whereas restoration increases it. Evolutionary dynamics in these sequences suggest that completion of the motif, combined with viral reassortment can contribute to pandemic risks. In summary, inhibition of the immediate anti-viral response is ‘pre-packaged’ in IAV in the sequences of vRNP-associated polymerase proteins.
Suggested Citation
Swantje Liedmann & Eike R. Hrincius & Cliff Guy & Darisuren Anhlan & Rüdiger Dierkes & Robert Carter & Gang Wu & Peter Staeheli & Douglas R. Green & Thorsten Wolff & Jonathan A. McCullers & Stephan Lu, 2014.
"Viral suppressors of the RIG-I-mediated interferon response are pre-packaged in influenza virions,"
Nature Communications, Nature, vol. 5(1), pages 1-8, December.
Handle:
RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6645
DOI: 10.1038/ncomms6645
Download full text from publisher
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6645. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.