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Independent recruitment of Igh alleles in V(D)J recombination

Author

Listed:
  • Clara F. Alves-Pereira

    (Epigenetics and Soma Laboratory, Instituto Gulbenkian de Ciência)

  • Raquel de Freitas

    (Epigenetics and Soma Laboratory, Instituto Gulbenkian de Ciência)

  • Telma Lopes

    (Flow Cytometry Laboratory UIC, Instituto Gulbenkian de Ciência)

  • Rui Gardner

    (Flow Cytometry Laboratory UIC, Instituto Gulbenkian de Ciência)

  • Filipa Marta

    (Epigenetics and Soma Laboratory, Instituto Gulbenkian de Ciência)

  • Paulo Vieira

    (Unité Lymphopoïèse, Institut Pasteur
    INSERM U668)

  • Vasco M. Barreto

    (Epigenetics and Soma Laboratory, Instituto Gulbenkian de Ciência)

Abstract

How the vast majority of B cells express only one of the two alleles at their immunoglobulin loci remains a biological puzzle. Here, in mice reconstituted with a single haematopoietic stem cell, we demonstrate that each of the two immunoglobulin heavy chain (Igh) alleles has a similar probability to be the first to undergo VH to DJH rearrangement. We also observe this similar probability in clones from multipotent and common lymphoid precursors. The extreme biases in the expression of the alleles that we find in more differentiated subsets are mostly due to constraints imposed by early rearrangements. Our data demonstrate that each of the two Igh alleles in a B cell behaves independently of the other, up to the moment when a successful rearrangement in one allele triggers a feedback mechanism that prevents further recombination.

Suggested Citation

  • Clara F. Alves-Pereira & Raquel de Freitas & Telma Lopes & Rui Gardner & Filipa Marta & Paulo Vieira & Vasco M. Barreto, 2014. "Independent recruitment of Igh alleles in V(D)J recombination," Nature Communications, Nature, vol. 5(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6623
    DOI: 10.1038/ncomms6623
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