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A haploid genetics toolbox for Arabidopsis thaliana

Author

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  • Maruthachalam Ravi

    (University of California
    School of Biology, Indian Institute of Science Education and Research, IISER-Thiruvananthapuram, CET Campus, Thiruvananthapuram 695016, India)

  • Mohan Prem Anand Marimuthu

    (University of California
    Genome Center, University of California)

  • Ek Han Tan

    (University of California
    Genome Center, University of California)

  • Shamoni Maheshwari

    (University of California
    Genome Center, University of California)

  • Isabelle M. Henry

    (University of California
    Genome Center, University of California)

  • Brenda Marin-Rodriguez

    (University of California)

  • Guillaume Urtecho

    (University of California)

  • Jie Tan

    (University of California)

  • Kristina Thornhill

    (University of California)

  • Fan Zhu

    (University of California)

  • Aneesh Panoli

    (University of California)

  • Venkatesan Sundaresan

    (University of California)

  • Anne B. Britt

    (University of California)

  • Luca Comai

    (University of California
    Genome Center, University of California)

  • Simon W. L. Chan

    (University of California
    Howard-Hughes Medical Institute and the Gordon and Betty Moore Foundation, University of California)

Abstract

Genetic analysis in haploids provides unconventional yet powerful advantages not available in diploid organisms. In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germination. We also show that haploids can be used as a tool to accelerate a variety of genetic analyses, specifically pyramiding multiple mutant combinations, forward mutagenesis screens, scaling down a tetraploid to lower ploidy levels and swapping of nuclear and cytoplasmic genomes. Furthermore, the A. thaliana HI can be used to produce haploids from a related species A. suecica and generate homozygous mutant plants from strong maternal gametophyte lethal alleles, which is not possible via conventional diploid genetics. Taken together, our results demonstrate the utility and power of haploid genetics in A. thaliana.

Suggested Citation

  • Maruthachalam Ravi & Mohan Prem Anand Marimuthu & Ek Han Tan & Shamoni Maheshwari & Isabelle M. Henry & Brenda Marin-Rodriguez & Guillaume Urtecho & Jie Tan & Kristina Thornhill & Fan Zhu & Aneesh Pan, 2014. "A haploid genetics toolbox for Arabidopsis thaliana," Nature Communications, Nature, vol. 5(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6334
    DOI: 10.1038/ncomms6334
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