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MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane

Author

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  • Yoshinori Sato

    (Molecular Cellular Biology Laboratory, Yokohama City University, Graduate School of Medical Science
    Yokohama City University, Graduate School of Medical Science)

  • Kenji Hayashi

    (Molecular Cellular Biology Laboratory, Yokohama City University, Graduate School of Medical Science)

  • Yoshiko Amano

    (Molecular Cellular Biology Laboratory, Yokohama City University, Graduate School of Medical Science)

  • Mikiko Takahashi

    (Faculty of Pharmaceutical Sciences, Teikyo Heisei University)

  • Shigenobu Yonemura

    (Electron Microscope Laboratory, RIKEN Center for Developmental Biology)

  • Ikuko Hayashi

    (Molecular Medical Bioscience Laboratory, Yokohama City University, Graduate School of Medical Life Science)

  • Hiroko Hirose

    (Yokohama City University, Graduate School of Medical Science)

  • Shigeo Ohno

    (Yokohama City University, Graduate School of Medical Science)

  • Atsushi Suzuki

    (Molecular Cellular Biology Laboratory, Yokohama City University, Graduate School of Medical Science)

Abstract

Recent studies have revealed the presence of a microtubule subpopulation called Golgi-derived microtubules that support Golgi ribbon formation, which is required for maintaining polarized cell migration. CLASPs and AKAP450/CG-NAP are involved in their formation, but the underlying molecular mechanisms remain unclear. Here, we find that the microtubule-crosslinking protein, MTCL1, is recruited to the Golgi membranes through interactions with CLASPs and AKAP450/CG-NAP, and promotes microtubule growth from the Golgi membrane. Correspondingly, MTCL1 knockdown specifically impairs the formation of the stable perinuclear microtubule network to which the Golgi ribbon tethers and extends. Rescue experiments demonstrate that besides its crosslinking activity mediated by the N-terminal microtubule-binding region, the C-terminal microtubule-binding region plays essential roles in these MTCL1 functions through a novel microtubule-stabilizing activity. These results suggest that MTCL1 cooperates with CLASPs and AKAP450/CG-NAP in the formation of the Golgi-derived microtubules, and mediates their development into a stable microtubule network.

Suggested Citation

  • Yoshinori Sato & Kenji Hayashi & Yoshiko Amano & Mikiko Takahashi & Shigenobu Yonemura & Ikuko Hayashi & Hiroko Hirose & Shigeo Ohno & Atsushi Suzuki, 2014. "MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane," Nature Communications, Nature, vol. 5(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6266
    DOI: 10.1038/ncomms6266
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