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A long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imaging

Author

Listed:
  • Jiahui Wu

    (University of Alberta)

  • Ahmed S. Abdelfattah

    (University of Alberta)

  • Loïs S. Miraucourt

    (Montreal Neurological Institute, Neuroengineering Program, McGill University)

  • Elena Kutsarova

    (Montreal Neurological Institute, Neuroengineering Program, McGill University)

  • Araya Ruangkittisakul

    (University of Alberta)

  • Hang Zhou

    (University of Alberta)

  • Klaus Ballanyi

    (University of Alberta)

  • Geoffrey Wicks

    (Montana State University)

  • Mikhail Drobizhev

    (Montana State University)

  • Aleksander Rebane

    (Montana State University
    National Institute of Chemical Physics and Biophysics)

  • Edward S. Ruthazer

    (Montreal Neurological Institute, Neuroengineering Program, McGill University)

  • Robert E. Campbell

    (University of Alberta)

Abstract

The introduction of calcium ion (Ca2+) indicators based on red fluorescent proteins (RFPs) has created new opportunities for multicolour visualization of intracellular Ca2+ dynamics. However, one drawback of these indicators is that they have optimal two-photon excitation outside the near-infrared window (650–1,000 nm) where tissue is most transparent to light. To address this shortcoming, we developed a long Stokes shift RFP-based Ca2+ indicator, REX-GECO1, with optimal two-photon excitation at

Suggested Citation

  • Jiahui Wu & Ahmed S. Abdelfattah & Loïs S. Miraucourt & Elena Kutsarova & Araya Ruangkittisakul & Hang Zhou & Klaus Ballanyi & Geoffrey Wicks & Mikhail Drobizhev & Aleksander Rebane & Edward S. Ruthaz, 2014. "A long Stokes shift red fluorescent Ca2+ indicator protein for two-photon and ratiometric imaging," Nature Communications, Nature, vol. 5(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6262
    DOI: 10.1038/ncomms6262
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