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An authentic imaging probe to track cell fate from beginning to end

Author

Listed:
  • Seung Koo Lee

    (Molecular Imaging Innovations Institute, Weill Cornell Medical College)

  • Luke J. Mortensen

    (Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School)

  • Charles P. Lin

    (Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School)

  • Ching-Hsuan Tung

    (Molecular Imaging Innovations Institute, Weill Cornell Medical College)

Abstract

Accurate tracing of cell viability is critical for optimizing delivery methods and evaluating the efficacy and safety of cell therapeutics. A nanoparticle-based cell tracker is developed to image cell fate from live to dead. The particle is fabricated from two types of optically quenched polyelectrolytes, a life indicator and a death indicator, through electrostatic interactions. On incubation with cells, the fabricated bifunctional nanoprobes are taken up efficiently and the first colour is produced by normal intracellular proteolysis, reflecting the healthy status of the cells. Depending on the number of coated layers, the signal can persist for several replication cycles. However, as the cells begin dying, the second colour appears quickly to reflect the new cell status. Using this chameleon-like cell tracker, live cells can be distinguished from apoptotic and necrotic cells instantly and definitively.

Suggested Citation

  • Seung Koo Lee & Luke J. Mortensen & Charles P. Lin & Ching-Hsuan Tung, 2014. "An authentic imaging probe to track cell fate from beginning to end," Nature Communications, Nature, vol. 5(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms6216
    DOI: 10.1038/ncomms6216
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