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Live imaging of calcium spikes during double fertilization in Arabidopsis

Author

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  • Yuki Hamamura

    (Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku
    JST ERATO Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, Chikusa-ku
    Present address: Institut de recherche en biologie végétale, Département de sciences biologiques, Université de Montréal, 4101 Rue Sherbrooke est, Montréal, Québec, Canada H1X 2B2)

  • Moe Nishimaki

    (Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku)

  • Hidenori Takeuchi

    (Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku
    JST ERATO Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, Chikusa-ku)

  • Anja Geitmann

    (Institut de recherche en biologie végétale, Université de Montréal)

  • Daisuke Kurihara

    (Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku
    JST ERATO Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, Chikusa-ku)

  • Tetsuya Higashiyama

    (Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku
    JST ERATO Higashiyama Live-Holonics Project, Nagoya University, Furo-cho, Chikusa-ku
    Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-cho, Chikusa-ku)

Abstract

Ca2+ waves and oscillation are key signalling elements during the fertilization process of animals, and are involved, for example, in egg activation. In the unique double fertilization process in flowering plants, both the egg cell and the neighbouring central cell fuse with a sperm cell each. Here we succeeded in imaging cytosolic Ca2+ in these two cells, and in the two synergid cells that accompany the gametes during semi-in vivo double fertilization. Following pollen tube discharge and plasmogamy, the egg and central cells displayed transient Ca2+ spikes, but not oscillations. Only the events in the egg cell correlated with the plasmogamy. In contrast, the synergid cells displayed Ca2+ oscillations on pollen tube arrival. The two synergid cells showed distinct Ca2+ dynamics depending on their respective roles in tube reception. These Ca2+ dynamics in the female gametophyte seem to represent highly specific signatures that coordinate successful double fertilization in the flowering plants.

Suggested Citation

  • Yuki Hamamura & Moe Nishimaki & Hidenori Takeuchi & Anja Geitmann & Daisuke Kurihara & Tetsuya Higashiyama, 2014. "Live imaging of calcium spikes during double fertilization in Arabidopsis," Nature Communications, Nature, vol. 5(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms5722
    DOI: 10.1038/ncomms5722
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